BRENDA - Enzyme Database
show all sequences of 1.14.11.55

Osmotically induced synthesis of the compatible solute hydroxyectoine is mediated by an evolutionarily conserved ectoine hydroxylase

Bursy, J.; Pierik, A.J.; Pica, N.; Bremer, E.; J. Biol. Chem. 282, 31147-31155 (2007)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ascorbate
5 mM, 70% inhibition
Virgibacillus salexigens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5
-
ectoine
pH 7.5, 32C
Virgibacillus salexigens
5.2
-
2-oxoglutarate
pH 7.5, 32C
Virgibacillus salexigens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
presence of Fe2+ is required
Virgibacillus salexigens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
-
Virgibacillus salexigens
34400
-
-
Virgibacillus salexigens
36000
-
gel filtration
Virgibacillus salexigens
Organism
Organism
UniProt
Commentary
Textmining
Virgibacillus salexigens
Q2TDY4
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Virgibacillus salexigens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.06
-
pH 7.5, 32C
Virgibacillus salexigens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ectoine + 2-oxoglutarate + O2
-
740684
Virgibacillus salexigens
5-hydroxyectoine + succinate + CO2
reaction is dependent on iron(II), molecular oxygen, and 2-oxoglutarate
-
-
?
additional information
the cosubstrate 2-oxoglutarate is stoichiometrically decarboxylated during the hydroxylation of the substrate ectoine. CO2 is thereby liberated from 2-oxoglutarate to form succinate. During the hydroxylation reaction, one atom of the atmospheric oxygen molecule is incorporated into succinate, whereas the other atom is incorporated into the hydroxy group formed on ectoine
740684
Virgibacillus salexigens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 34000, SDS-PAGE, 1 * 34400, mass spectrometry
Virgibacillus salexigens
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
32
-
-
Virgibacillus salexigens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Virgibacillus salexigens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ascorbate
5 mM, 70% inhibition
Virgibacillus salexigens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5
-
ectoine
pH 7.5, 32C
Virgibacillus salexigens
5.2
-
2-oxoglutarate
pH 7.5, 32C
Virgibacillus salexigens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
presence of Fe2+ is required
Virgibacillus salexigens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
-
Virgibacillus salexigens
34400
-
-
Virgibacillus salexigens
36000
-
gel filtration
Virgibacillus salexigens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Virgibacillus salexigens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.06
-
pH 7.5, 32C
Virgibacillus salexigens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ectoine + 2-oxoglutarate + O2
-
740684
Virgibacillus salexigens
5-hydroxyectoine + succinate + CO2
reaction is dependent on iron(II), molecular oxygen, and 2-oxoglutarate
-
-
?
additional information
the cosubstrate 2-oxoglutarate is stoichiometrically decarboxylated during the hydroxylation of the substrate ectoine. CO2 is thereby liberated from 2-oxoglutarate to form succinate. During the hydroxylation reaction, one atom of the atmospheric oxygen molecule is incorporated into succinate, whereas the other atom is incorporated into the hydroxy group formed on ectoine
740684
Virgibacillus salexigens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 34000, SDS-PAGE, 1 * 34400, mass spectrometry
Virgibacillus salexigens
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
32
-
-
Virgibacillus salexigens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Virgibacillus salexigens
Expression
Organism
Commentary
Expression
Virgibacillus salexigens
ectoine production is linearly correlated with the salinity of the growth medium. The formation of 5-hydroxyectoine is primarily a stationary growth phase phenomenon. The transcript levels of the ectABC and ectD genes increase as a function of salinity
additional information
Expression (protein specific)
Organism
Commentary
Expression
Virgibacillus salexigens
ectoine production is linearly correlated with the salinity of the growth medium. The formation of 5-hydroxyectoine is primarily a stationary growth phase phenomenon. The transcript levels of the ectABC and ectD genes increase as a function of salinity
additional information
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741719
Tao
Ectoine and 5-hydroxyectoine ...
Virgibacillus halodenitrificans, Virgibacillus halodenitrificans PDB-F2
Appl. Microbiol. Biotechnol.
100
6779-6789
2016
-
-
-
-
-
-
-
-
-
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-
2
-
2
-
-
-
-
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2
-
1
-
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-
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-
2
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-
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-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
742383
Widderich
Strangers in the archaeal wor ...
Nitrosopumilus maritimus
Environ. Microbiol.
18
1227-1248
2016
-
-
1
-
-
-
-
-
-
-
1
1
-
7
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
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-
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-
-
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-
-
1
-
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-
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-
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-
1
1
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-
-
1
-
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-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741466
Yang
Crystallization and X-ray diff ...
Bacillus pseudofirmus, Bacillus pseudofirmus OF4
Wei Sheng Wu Xue Bao
55
1468-1474
2015
-
-
1
1
-
-
-
-
-
-
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-
5
-
-
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1
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1
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1
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-
-
-
-
-
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-
739813
Hoeppner
Overexpression, crystallizatio ...
Sphingopyxis alaskensis
Acta Crystallogr. Sect. F
70
493-496
2014
-
-
1
1
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
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1
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1
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1
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-
-
740729
Hoeppner
Crystal structure of the ectoi ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593
J. Biol. Chem.
289
29570-29583
2014
-
-
-
1
7
-
-
2
-
-
2
-
-
5
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
1
7
-
-
-
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2
-
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2
-
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2
1
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-
740868
Widderich
Molecular dynamics simulations ...
Virgibacillus salexigens
J. Mol. Biol.
426
586-600
2014
-
-
-
1
36
-
-
6
-
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3
-
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1
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1
36
-
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6
-
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-
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-
1
-
-
-
-
-
-
-
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-
-
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741304
Widderich
Biochemical properties of ecto ...
Acidiphilium cryptum, Alkalilimnicola ehrlichii, Halomonas elongata, Halomonas elongata DSM 2581, Paenibacillus lautus, Pseudomonas stutzeri, Pseudomonas stutzeri A1501, Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593, Virgibacillus salexigens
PLoS ONE
9
e93809
2014
-
-
-
1
-
-
7
14
-
21
7
20
-
17
-
-
-
-
-
-
-
-
20
7
8
7
7
-
7
7
7
-
-
-
7
-
-
-
-
-
1
-
-
-
7
-
14
-
21
7
20
-
-
-
-
-
-
-
-
20
7
7
7
-
7
7
7
-
7
-
-
-
-
7
7
743087
Widderich
Molecular dynamics simulation ...
Virgibacillus salexigens
J. Mol. Biol.
426
586-600
2014
-
-
1
-
36
-
-
14
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
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-
-
-
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1
-
-
36
-
-
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-
14
-
1
-
1
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-
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-
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
740784
Eilert
Synthesis and release of the b ...
Halomonas elongata, Halomonas elongata DSM 2581
J. Biotechnol.
167
85-93
2013
-
1
-
-
-
-
-
-
-
-
-
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3
-
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-
-
-
-
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-
1
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-
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1
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-
-
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-
741265
Reuter
Synthesis of 5-hydroxyectoine ...
Virgibacillus salexigens
PLoS ONE
5
e10647
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
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-
-
-
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1
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1
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-
739899
Bursy
Synthesis and uptake of the co ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Appl. Environ. Microbiol.
74
7286-7296
2008
-
-
-
-
-
-
1
2
-
1
1
-
-
43
-
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1
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1
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4
1
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1
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1
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1
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2
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1
1
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1
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1
-
4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
740684
Bursy
Osmotically induced synthesis ...
Virgibacillus salexigens
J. Biol. Chem.
282
31147-31155
2007
-
-
-
-
-
-
1
2
-
1
3
-
-
6
-
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1
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1
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2
1
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1
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1
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1
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2
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1
3
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1
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1
-
2
1
1
-
-
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1
-
-
-
1
-
-
1
-
-
739896
Prabhu
Functional expression of the e ...
Streptomyces anulatus
Appl. Environ. Microbiol.
70
3130-3132
2004
-
1
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3
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1
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1
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1
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1
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