BRENDA - Enzyme Database
show all sequences of 1.14.11.55

Synthesis and uptake of the compatible solutes ectoine and 5-hydroxyectoine by Streptomyces coelicolor A3(2) in response to salt and heat stresses

Bursy, J.; Kuhlmann, A.U.; Pittelkow, M.; Hartmann, H.; Jebbar, M.; Pierik, A.J.; Bremer, E.; Appl. Environ. Microbiol. 74, 7286-7296 (2008)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Fe2+
stimulation at 1 mM, inhibitory above
Streptomyces coelicolor
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6
-
ectoine
pH not specified in the publication, temperature not specified in the publication
Streptomyces coelicolor
6.2
-
2-oxoglutarate
pH not specified in the publication, temperature not specified in the publication
Streptomyces coelicolor
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
stimulation at 1 mM, inhibitory above
Streptomyces coelicolor
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38500
-
-
Streptomyces coelicolor
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor A3(2)
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Streptomyces coelicolor
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
20
-
pH not specified in the publication, temperature not specified in the publication
Streptomyces coelicolor
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ectoine + 2-oxoglutarate + O2
-
739899
Streptomyces coelicolor
5-hydroxyectoine + succinate + CO2
-
-
-
?
ectoine + 2-oxoglutarate + O2
-
739899
Streptomyces coelicolor A3(2)
5-hydroxyectoine + succinate + CO2
-
-
-
?
additional information
no substrate: L-proline
739899
Streptomyces coelicolor
?
-
-
-
-
additional information
no substrate: L-proline
739899
Streptomyces coelicolor A3(2)
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 38500, SDS-PAGE, x * 32833, calculated
Streptomyces coelicolor
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
-
Streptomyces coelicolor
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptomyces coelicolor
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Fe2+
stimulation at 1 mM, inhibitory above
Streptomyces coelicolor
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.6
-
ectoine
pH not specified in the publication, temperature not specified in the publication
Streptomyces coelicolor
6.2
-
2-oxoglutarate
pH not specified in the publication, temperature not specified in the publication
Streptomyces coelicolor
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
stimulation at 1 mM, inhibitory above
Streptomyces coelicolor
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38500
-
-
Streptomyces coelicolor
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces coelicolor
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
20
-
pH not specified in the publication, temperature not specified in the publication
Streptomyces coelicolor
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ectoine + 2-oxoglutarate + O2
-
739899
Streptomyces coelicolor
5-hydroxyectoine + succinate + CO2
-
-
-
?
ectoine + 2-oxoglutarate + O2
-
739899
Streptomyces coelicolor A3(2)
5-hydroxyectoine + succinate + CO2
-
-
-
?
additional information
no substrate: L-proline
739899
Streptomyces coelicolor
?
-
-
-
-
additional information
no substrate: L-proline
739899
Streptomyces coelicolor A3(2)
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38500, SDS-PAGE, x * 32833, calculated
Streptomyces coelicolor
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
-
-
Streptomyces coelicolor
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptomyces coelicolor
General Information
General Information
Commentary
Organism
physiological function
the accumulation of the compatible solutes ectoine and 5-hydroxyectoine allows Streptomyces coelicolor A3(2) to fend off the detrimental effects of both high salinity and high temperature on cell physiology
Streptomyces coelicolor
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the accumulation of the compatible solutes ectoine and 5-hydroxyectoine allows Streptomyces coelicolor A3(2) to fend off the detrimental effects of both high salinity and high temperature on cell physiology
Streptomyces coelicolor
Other publictions for EC 1.14.11.55
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741719
Tao
Ectoine and 5-hydroxyectoine ...
Virgibacillus halodenitrificans, Virgibacillus halodenitrificans PDB-F2
Appl. Microbiol. Biotechnol.
100
6779-6789
2016
-
-
-
-
-
-
-
-
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2
-
2
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-
-
-
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2
-
1
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2
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-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
742383
Widderich
Strangers in the archaeal wor ...
Nitrosopumilus maritimus
Environ. Microbiol.
18
1227-1248
2016
-
-
1
-
-
-
-
-
-
-
1
1
-
7
-
-
1
-
-
-
-
-
1
1
1
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-
-
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-
-
-
-
-
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-
-
1
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-
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-
-
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-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741466
Yang
Crystallization and X-ray diff ...
Bacillus pseudofirmus, Bacillus pseudofirmus OF4
Wei Sheng Wu Xue Bao
55
1468-1474
2015
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
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-
-
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-
-
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-
1
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1
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1
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-
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-
-
-
-
-
-
-
-
-
-
739813
Hoeppner
Overexpression, crystallizatio ...
Sphingopyxis alaskensis
Acta Crystallogr. Sect. F
70
493-496
2014
-
-
1
1
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
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-
-
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-
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1
-
1
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1
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-
-
-
740729
Hoeppner
Crystal structure of the ectoi ...
Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593
J. Biol. Chem.
289
29570-29583
2014
-
-
-
1
7
-
-
2
-
-
2
-
-
5
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
7
-
-
-
-
2
-
-
2
-
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-
-
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-
-
-
2
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
740868
Widderich
Molecular dynamics simulations ...
Virgibacillus salexigens
J. Mol. Biol.
426
586-600
2014
-
-
-
1
36
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
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-
1
36
-
-
-
-
6
-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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741304
Widderich
Biochemical properties of ecto ...
Acidiphilium cryptum, Alkalilimnicola ehrlichii, Halomonas elongata, Halomonas elongata DSM 2581, Paenibacillus lautus, Pseudomonas stutzeri, Pseudomonas stutzeri A1501, Sphingopyxis alaskensis, Sphingopyxis alaskensis DSM 13593, Virgibacillus salexigens
PLoS ONE
9
e93809
2014
-
-
-
1
-
-
7
14
-
21
7
20
-
17
-
-
-
-
-
-
-
-
20
7
8
7
7
-
7
7
7
-
-
-
7
-
-
-
-
-
1
-
-
-
7
-
14
-
21
7
20
-
-
-
-
-
-
-
-
20
7
7
7
-
7
7
7
-
7
-
-
-
-
7
7
743087
Widderich
Molecular dynamics simulation ...
Virgibacillus salexigens
J. Mol. Biol.
426
586-600
2014
-
-
1
-
36
-
-
14
-
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
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1
-
-
36
-
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-
-
14
-
1
-
1
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-
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-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740784
Eilert
Synthesis and release of the b ...
Halomonas elongata, Halomonas elongata DSM 2581
J. Biotechnol.
167
85-93
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
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-
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1
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1
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-
741265
Reuter
Synthesis of 5-hydroxyectoine ...
Virgibacillus salexigens
PLoS ONE
5
e10647
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
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-
-
-
-
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1
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1
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-
739899
Bursy
Synthesis and uptake of the co ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Appl. Environ. Microbiol.
74
7286-7296
2008
-
-
-
-
-
-
1
2
-
1
1
-
-
43
-
-
1
-
-
-
1
-
4
1
-
1
-
-
-
1
-
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-
1
-
2
-
1
1
-
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1
-
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1
-
4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
740684
Bursy
Osmotically induced synthesis ...
Virgibacillus salexigens
J. Biol. Chem.
282
31147-31155
2007
-
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1
2
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1
3
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6
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1
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1
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2
1
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1
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1
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1
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2
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1
3
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1
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1
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2
1
1
-
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-
1
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-
1
-
-
1
-
-
739896
Prabhu
Functional expression of the e ...
Streptomyces anulatus
Appl. Environ. Microbiol.
70
3130-3132
2004
-
1
-
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-
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3
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1
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1
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1
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1
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