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Literature summary for 1.14.11.45 extracted from

  • Zhang, C.; Liu, Y.; Xue, N.; Wang, X.; Xie, X.; Xu, Q.; Chen, N.
    Characterization of recombinant L-isoleucine-4-hydroxylase from Bacillus thuringiensis and its application in 4-hydroxyisoleucine biosynthesis (2014), Wei Sheng Wu Xue Bao, 54, 889-896 .
    View publication on PubMed
Search for more literature for 1.14.11.45

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 L-isoleucine at pH 7.0 and 35°C Bacillus thuringiensis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2 Bacillus thuringiensis
-
 (4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
 ?
L-isoleucine + 2-oxoglutarate + O2 Bacillus thuringiensis TCCC 11826
-
 (4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis S5SPJ1
-
-
Bacillus thuringiensis TCCC 11826 S5SPJ1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2
-
 Bacillus thuringiensis (4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
 ?
L-isoleucine + 2-oxoglutarate + O2
-
 Bacillus thuringiensis TCCC 11826 (4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
IDO
-
 Bacillus thuringiensis
L-isoleucine dioxygenase
-
 Bacillus thuringiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Bacillus thuringiensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 the relative activity of the enzyme remains 85.1% after 5 h incubation at 35°C Bacillus thuringiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus thuringiensis