Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta2(DE3) cells | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
2-oxoglutarate | at pH 6.0 and 25°C | Bacillus thuringiensis | |
0.27 | - |
L-isoleucine | at pH 6.0 and 25°C | Bacillus thuringiensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3R,4S)-4-hydroxy-L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis | - |
(2S,3R)-2-amino-3-methyl-4-ketopentanoate + succinate + CO2 | - |
? | |
(2S,3R,4S)-4-hydroxy-L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis 2e2 | - |
(2S,3R)-2-amino-3-methyl-4-ketopentanoate + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis | best substrate | (2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis 2e2 | best substrate | (2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
- |
- |
Bacillus thuringiensis 2e2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3R,4S)-4-hydroxy-L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | (2S,3R)-2-amino-3-methyl-4-ketopentanoate + succinate + CO2 | - |
? | |
(2S,3R,4S)-4-hydroxy-L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2e2 | (2S,3R)-2-amino-3-methyl-4-ketopentanoate + succinate + CO2 | - |
? | |
(S)-4-hydroxy-L-norvaline + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | ? | - |
? | |
(S)-4-hydroxy-L-norvaline + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2e2 | ? | - |
? | |
4-hydroxy-L-norleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | ? | - |
? | |
4-hydroxy-L-norleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2e2 | ? | - |
? | |
DL-homocysteine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | ? | - |
? | |
L-allo-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | (S)-3-hydroxy-L-allo-isoleucine + succinate + CO2 | - |
? | |
L-cysteine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | ? | - |
? | |
L-ethionine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | L-ethionine sulfoxide + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | best substrate | Bacillus thuringiensis | (2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | best substrate | Bacillus thuringiensis 2e2 | (2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-leucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | 4-hydroxy-L-leucine + succinate + CO2 | - |
? | |
L-methionine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | L-methionine sulfoxide + succinate + CO2 | - |
? | |
L-norleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | 4-hydroxy-L-norleucine + succinate + CO2 | - |
? | |
L-norvaline + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | (S)-4-hydroxy-L-norvaline + succinate + CO2 | - |
? | |
additional information | the enzyme does not react with D-Ile, D-allo-Ile, tert-butoxycarbonyl-L-Ile, 9-fluorenylmethoxycarbonyl-L-Ile,N-carbamoyl-L-Ile, L-Ile methyl ester, and N-acetyl-L-Ile methyl ester | Bacillus thuringiensis | ? | - |
? | |
additional information | the enzyme does not react with D-Ile, D-allo-Ile, tert-butoxycarbonyl-L-Ile, 9-fluorenylmethoxycarbonyl-L-Ile,N-carbamoyl-L-Ile, L-Ile methyl ester, and N-acetyl-L-Ile methyl ester | Bacillus thuringiensis 2e2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDO | - |
Bacillus thuringiensis |
L-isoleucine dioxygenase | - |
Bacillus thuringiensis |