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Literature summary for 1.14.11.43 extracted from

  • Müller, T.A.; Fleischmann, T.; van der Meer, J.R.; Kohler, H.P.
    Purification and characterization of two enantioselective alpha-ketoglutarate-dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH (2006), Appl. Environ. Microbiol., 72, 4853-4861.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
ascorbate slightly reduced in the absence of ascorbate Sphingobium herbicidovorans

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His6-tagged fusion protein from Escherichia coli BL21(DE3)(pLysS) Sphingobium herbicidovorans

General Stability

General Stability Organism
storage on ice and freeze-thaw cycles reduce the activity to about 80% of the original activity Sphingobium herbicidovorans

Inhibitors

Inhibitors Comment Organism Structure
(S)-2-(2,4-dichlorophenoxy)propanoate slight inhibition Sphingobium herbicidovorans
(S)-2-(4-chloro-2-methylphenoxy)propanoate slight inhibition Sphingobium herbicidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0196
-
 2-oxoglutarate pH 6.8, 30°C Sphingobium herbicidovorans
0.132
-
 (S)-2-(4-chloro-2-methylphenoxy)propanoate pH 6.8, 30°C Sphingobium herbicidovorans
0.159
-
 O2 pH 6.8, 30°C, above 0.23 mM Sphingobium herbicidovorans
0.495
-
 (S)-(2,4-dichlorophenoxy)propanoate pH 6.8, 30°C Sphingobium herbicidovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ ferrous iron is necessary for activity of the enzyme and other divalent cations could not replace it Sphingobium herbicidovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 gel filtration Sphingobium herbicidovorans
33700
-
 1 * 33700, calculated from sequence Sphingobium herbicidovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 Sphingobium herbicidovorans i.e. (S)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate 2,4-dichlorophenol + pyruvate + succinate + CO2
-
 ?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 Sphingobium herbicidovorans i.e. (S)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate 2-methyl-4-chlorophenol + pyruvate + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingobium herbicidovorans Q700X4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingobium herbicidovorans

Storage Stability

Storage Stability Organism
4°C, 3 days, less than 10% loss of activity Sphingobium herbicidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 i.e. (S)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate Sphingobium herbicidovorans 2,4-dichlorophenol + pyruvate + succinate + CO2
-
 ?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 i.e. (S)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate Sphingobium herbicidovorans 2-methyl-4-chlorophenol + pyruvate + succinate + CO2
-
 ?
(S)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2 i.e. (S)-dichlorprop. SdpA is specific for the (S) enantiomer Sphingobium herbicidovorans 2,4-dichlorophenol + pyruvate + succinate + CO2
-
 ?
(S)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2 i.e. (S)-mecoprop. SdpA is specific for the (S) enantiomer. The enzyme is specific for 2-oxoglutarate. Low levels of activity (1.9%) in presence of 2-oxoadipate. No activity with oxaloacetate, 2-oxobutyrate, and 2-oxovalerate Sphingobium herbicidovorans 4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Sphingobium herbicidovorans
monomer 1 * 33700, calculated from sequence Sphingobium herbicidovorans

Synonyms

Synonyms Comment Organism
2-oxoglutarate-dependent (S)-dichlorprop dioxygenase
-
 Sphingobium herbicidovorans
alpha-ketoglutarate-dependent (S)-dichlorprop dioxygenase
-
 Sphingobium herbicidovorans
SdpA
-
 Sphingobium herbicidovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Sphingobium herbicidovorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 20°C: about 40% of maximal activity, 35-40°C: optimal activity Sphingobium herbicidovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 stable for several hours Sphingobium herbicidovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Sphingobium herbicidovorans

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 pH 6.0: about 30% of maximal activity, pH 7.5: about 50% of maximal activity Sphingobium herbicidovorans

General Information

General Information Comment Organism
physiological function the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH Sphingobium herbicidovorans