Cloned (Comment) | Organism |
---|---|
expression of 40 amino acid residues of the C-terminal end of isozyme LH1 fused to human cathepsin D and c-Myc-tagged and 2 mutant variants thereof in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K694G | site-directed mutagenesis, point mutation is introduced in the LH1 part of the expression construct comprising 40 amino acid residues of the C-terminal end of isozyme LH1, responsible for endoplasmic reticulum localization, fused to human cathepsin D and c-Myc-tagged, the exchange of the charged residue and deletion of 8 amino acids of the last 40 residues at the enzymes' C-terminal end has no effect on retention efficiency of the reporter protein, but deletion of the next 8 amino acid residues, leaving 24 residues, increases the secretion level of enzyme from the cell | Homo sapiens |
R693Q | site-directed mutagenesis, point mutation is introduced in the LH1 part of the expression construct comprising 40 amino acid residues of the C-terminal end of isozyme LH1, responsible for endoplasmic reticulum localization, fused to human cathepsin D and c-Myc-tagged, the exchange of the charged residue and deletion of 8 amino acis of the last 40 residues at the enzymes' C-terminal end has no effect on retention efficiency of the reporter protein, but deletion of the next 8 amino acid residues, leaving 24 residues, increases the secretion level of enzyme from the cell | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | lumen | Homo sapiens | 5783 | - |
membrane | peripheral | Homo sapiens | 16020 | - |
additional information | a peptide fold in the iron-binding site, within the 40 amino acid residues at the C-terminus, is responsible for KDEL-receptor-independent localization in the endoplasmic reticulum | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a peptide fold in the iron-binding site, within the 40 amino acid residues at the C-terminus, is responsible for KDEL-receptor-independent localization in the endoplasmic reticulum | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-lysine + 2-oxoglutarate + O2 | Homo sapiens | hydroxylation of lysine residues in collagenous sequences | procollagen 5-hydroxy-L-lysine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
3 isozyme LH1-3 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-lysine + 2-oxoglutarate + O2 | - |
Homo sapiens | procollagen 5-hydroxy-L-lysine + succinate + CO2 | - |
? | |
procollagen L-lysine + 2-oxoglutarate + O2 | hydroxylation of lysine residues in collagenous sequences | Homo sapiens | procollagen 5-hydroxy-L-lysine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LH | - |
Homo sapiens |
lysyl hydroxylase | - |
Homo sapiens |