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Literature summary for 1.14.11.37 extracted from
- The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the alpha-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK (2012), Angew. Chem. Int. Ed. Engl., 51, 3428-3431.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | dependent on | Streptomyces kanamyceticus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme is dependent on exogenous ferrous ions, although residual activity is also observed in the absence of exogenous Fe2+ | Streptomyces kanamyceticus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kanamycin B + 2-oxoglutarate + O2 | Streptomyces kanamyceticus | - |
2'-dehydrokanamycin A + succinate + NH3 + CO2 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces kanamyceticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-affinity column chromatography | Streptomyces kanamyceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kanamycin B + 2-oxoglutarate + O2 | - |
Streptomyces kanamyceticus | 2'-dehydrokanamycin A + succinate + NH3 + CO2 | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| kanJ | - |
Streptomyces kanamyceticus |
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Release 2023.1 (January 2023)
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