Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | blueberry scorch virus |
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | Blackberry virus Y |
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | grapevine virus A |
Protein Variants | Comment | Organism |
---|---|---|
D61A | the mutant is unable to rescue methylated RNA phage MS2 and is unable to convert 2-oxoglutarate to succinate | Blackberry virus Y |
H59A | the mutant is unable to rescue methylated RNA phage MS2 and is unable to convert 2-oxoglutarate to succinate | Blackberry virus Y |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Escherichia coli | |
Fe2+ | required for activity | blueberry scorch virus | |
Fe2+ | required for activity | Blackberry virus Y | |
Fe2+ | required for activity | grapevine virus A |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blackberry virus Y | - |
- |
- |
blueberry scorch virus | - |
- |
- |
Escherichia coli | - |
- |
- |
grapevine virus A | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | blueberry scorch virus |
TALON metal affinity resin column chromatography | Blackberry virus Y |
TALON metal affinity resin column chromatography | grapevine virus A |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
blueberry scorch virus | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Blackberry virus Y | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
grapevine virus A | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
blueberry scorch virus | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Blackberry virus Y | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
grapevine virus A | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
methylated DNA bacteriophage M13mp18 + 2-oxoglutarate + O2 | - |
blueberry scorch virus | DNA bacteriophage M13mp18 + formaldehyde + succinate + CO2 | - |
? | |
methylated DNA bacteriophage M13mp18 + 2-oxoglutarate + O2 | - |
Blackberry virus Y | DNA bacteriophage M13mp18 + formaldehyde + succinate + CO2 | - |
? | |
methylated DNA bacteriophage M13mp18 + 2-oxoglutarate + O2 | - |
grapevine virus A | DNA bacteriophage M13mp18 + formaldehyde + succinate + CO2 | - |
? | |
methylated RNA bacteriophage MS2 + 2-oxoglutarate + O2 | - |
blueberry scorch virus | RNA bacteriophage MS2 + formaldehyde + succinate + CO2 | - |
? | |
methylated RNA bacteriophage MS2 + 2-oxoglutarate + O2 | - |
Blackberry virus Y | RNA bacteriophage MS2 + formaldehyde + succinate + CO2 | - |
? | |
methylated RNA bacteriophage MS2 + 2-oxoglutarate + O2 | - |
grapevine virus A | RNA bacteriophage MS2 + formaldehyde + succinate + CO2 | - |
? | |
additional information | Escherichia coli AlkB is approximately 10fold more active on single-stranded DNA compared to single-stranded RNA, Eschichia coli AlkB displays a somewhat lower activity on double-stranded RNA substrates than on single-stranded RNA substrates | Escherichia coli | ? | - |
? | |
additional information | the viral AlkB protein prefers single-stranded RNA over single-stranded DNA substrates (10fold) | blueberry scorch virus | ? | - |
? | |
additional information | the viral AlkB protein prefers single-stranded RNA over single-stranded DNA substrates (10fold) | Blackberry virus Y | ? | - |
? | |
additional information | the viral AlkB protein prefers single-stranded RNA over single-stranded DNA substrates (10fold) | grapevine virus A | ? | - |
? | |
RNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | RNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
RNA-1-methyladenine + 2-oxoglutarate + O2 | - |
blueberry scorch virus | RNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
RNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Blackberry virus Y | RNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
RNA-1-methyladenine + 2-oxoglutarate + O2 | - |
grapevine virus A | RNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
RNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli | RNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
RNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
blueberry scorch virus | RNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
RNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Blackberry virus Y | RNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
RNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
grapevine virus A | RNA-cytosine + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlkB | - |
Escherichia coli |
AlkB | - |
blueberry scorch virus |
AlkB | - |
Blackberry virus Y |
AlkB | - |
grapevine virus A |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Escherichia coli | |
2-oxoglutarate | - |
blueberry scorch virus | |
2-oxoglutarate | - |
Blackberry virus Y | |
2-oxoglutarate | - |
grapevine virus A |
General Information | Comment | Organism |
---|---|---|
physiological function | viral AlkB proteins maintain the integrity of the viral RNA genome through repair of deleterious methylation damage | blueberry scorch virus |
physiological function | viral AlkB proteins maintain the integrity of the viral RNA genome through repair of deleterious methylation damage | Blackberry virus Y |
physiological function | viral AlkB proteins maintain the integrity of the viral RNA genome through repair of deleterious methylation damage | grapevine virus A |