Literature summary for 1.14.11.33 extracted from

Mishina, Y.; Chen, L.X.; He, C.
Preparation and characterization of the native iron(II)-containing DNA repair AlkB protein directly from Escherichia coli (2004), J. Am. Chem. Soc., 126, 16930-16936.

Search for more literature for 1.14.11.33

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D133C	the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme	Escherichia coli
H131C	the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme	Escherichia coli
H187C	the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the enzyme contains iron(II)	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA-base-CH3 + 2-oxoglutarate + O2	Escherichia coli	-	DNA-base + formaldehyde + succinate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-cellulose column chromatography, SP Sepharose cation exchange column chromatography, and Mono-S cation exchange column chromatography	Escherichia coli

Storage Stability

Storage Stability	Organism
the iron(II)-bound AlkB is reasonably stable at 4°C in the presence of 10 mM 2-mercaptoethanol at concentrations up to 2 mM	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA-1-methyladenine + 2-oxoglutarate + O2	-	Escherichia coli	DNA-adenine + formaldehyde + succinate + CO2	-	?
DNA-3-methylcytosine + 2-oxoglutarate + O2	-	Escherichia coli	DNA-cytosine + formaldehyde + succinate + CO2	-	?
DNA-base-CH3 + 2-oxoglutarate + O2	-	Escherichia coli	DNA-base + formaldehyde + succinate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
AlkB	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
2-oxoglutarate	-	Escherichia coli

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Release 2023.1 (January 2023)