Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 | Homo sapiens | - |
hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene fih | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-375 cell | melanoma cells | Homo sapiens | - |
LS-174T cell | colon adenocarcinoma cells | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 | - |
Homo sapiens | hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
asparaginyl hydroxylase | - |
Homo sapiens |
factor-inhibiting HIF | - |
Homo sapiens |
factor-inhibiting hypoxia-inducible factor | - |
Homo sapiens |
FIH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | FIH silencing leads to spacial displacement of the expression pattern of HIF target genes that depend on the C-TAD, such as carbonic anhydrase IX, to more oxygenated areas, whereas overexpression of FIH restricts this pattern to more hypoxic areas. Silencing of gene fih severely reduced in vitro cell proliferation and in vivo tumor growth of LS174 colon adenocarcinoma and A375 melanoma cells. Silencing of fih also significantly increases both the total and phosphorylated forms of the tumor suppressor p53, leading to an increase in its direct target, the cell cycle inhibitor p21. p53-deficient or mutant cells are totally insensitive to FIH expression. FIH activity is essential for tumor growth through the suppression of the p53-p21 axis, the major barrier that prevents cancer progression | Homo sapiens |
physiological function | FIH modulates the profile of hypoxia-inducible factor downstream genes and of hypoxia-inducible factor target genes in a physiological oxygen gradient: the in vitro spheroid model, overview. FIH enhances tumorigenesis and controls in vitro p53 expression. Factor-inhibiting hypoxia-inducible factor monitors the expression of a spectrum of genes that are dictated by the cell's partial oxygen pressure. This action is mediated by the C-TAD, one of two transactivation domains of the hypoxia-inducible factor | Homo sapiens |