Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-ascorbate | 2 mM, no further stimulation above 2 mM | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Citric acid | 30% inhibition at 5 mM | Streptomyces sp. | |
Co2+ | more than 90% inhibition at 1 mM | Streptomyces sp. | |
Cu2+ | more than 90% inhibition at 1 mM | Streptomyces sp. | |
EDTA | 95% inhibition at 2 mM | Streptomyces sp. | |
Zn2+ | more than 90% inhibition at 1 mM | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
2-oxoglutarate | 100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C | Streptomyces sp. | |
0.56 | - |
L-proline | 100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | strictly required, optimal stimulation at 1 mM | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
strainTH1 | - |
Streptomyces sp. TH1 | - |
strainTH1 | - |
Purification (Comment) | Organism |
---|---|
5 chromatographic steps near to homogeneity | Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Streptomyces sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
proline 3-hydroxylase | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
L-proline | 100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | strictly required, highest activity at 4 mM | Streptomyces sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces sp. | - |
- |
4.3 |