Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-2-nitrobenzoic acid | 1 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
Ca2+ | weak | Acremonium chrysogenum | |
EDTA | 0.6 mM, 83% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
iodoacetic acid | 1 mM, 2% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
N-ethylmaleimide | 1 mM, 97% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
o-phenanthroline | 0.6 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
p-hydroxymercuribenzoate | 1 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
Zn2+ | strong | Acremonium chrysogenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
deacetoxycephalosporin C | pH 7.5, 36°C | Acremonium chrysogenum | |
0.022 | - |
2-oxoglutarate | pH 7.5, 36°C | Acremonium chrysogenum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for both activities | Acremonium chrysogenum | |
additional information | Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+ | Acremonium chrysogenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
1 * 41000, SDS-PAGE | Acremonium chrysogenum |
43000 | - |
gel filtration | Acremonium chrysogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium chrysogenum | - |
catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.127 | - |
pH 7.5, 36°C | Acremonium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetylcephalosporin C + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Acremonium chrysogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | 38 | - |
Acremonium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Acremonium chrysogenum | isoelectric focusing | - |
6.3 |