Protein Variants | Comment | Organism |
---|---|---|
C162L | isozyme CAS2, site-directed mutagenesis, same activity in hydroxylation of N-alpha-acetyl-L-arginine as the wild-type enzyme | Streptomyces clavuligerus |
C200L | isozyme CAS2, site-directed mutagenesis, 42% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
C201L | isozyme CAS2, site-directed mutagenesis, 40% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
C8L | isozyme CAS2, site-directed mutagenesis, 42% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
H109L | isozyme CAS2, site-directed mutagenesis, 86% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
H122L | isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
H131L | isozyme CAS2, site-directed mutagenesis, 36% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
H145E | isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
H145L | isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
H145Q | site-directed mutagenesis, no activity in hydroxylation of N-alpha-acetyl-L-arginine | Streptomyces clavuligerus |
H151l | isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
H167L | isozyme CAS2, site-directed mutagenesis, 19% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
H280L | isozyme CAS2, site-directed mutagenesis, 86% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
H297L | isozyme CAS2, site-directed mutagenesis, 3% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
Q154L | isozyme CAS2, site-directed mutagenesis, 23% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme | Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Streptomyces clavuligerus | |
Fe2+ | H145 forms part of the HXE iron-binding motif in isozyme CAS2 | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
isozyme CAS2 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus | |
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | hydroxylation | Streptomyces clavuligerus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
dihydroclavaminate + 2-oxoglutarate + O2 | cyclization | Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 | hydroxylation | Streptomyces clavuligerus | 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O | - |
? | |
N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 | isozyme CAS2 | Streptomyces clavuligerus | 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | saturation | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CAS | - |
Streptomyces clavuligerus |