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Literature summary for 1.14.11.2 extracted from

  • Kersteen, E.A.; Higgin, J.J.; Raines, R.T.
    Production of human prolyl 4-hydroxylase in Escherichia coli (2004), Protein Expr. Purif., 38, 279-291.
    View publication on PubMed
Search for more literature for 1.14.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 (Pro-Pro-Gly)10
-
 Homo sapiens
0.078
-
 dansyl-Gly-Phe-Pro-Gly-OEt
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum lumen Homo sapiens 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
228000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
procollagen L-proline + 2-oxoglutarate + O2 Homo sapiens
-
 procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant P4H, poly(proline)-affinity column, anion-exchange, gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2300
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)10 + 2-oxoglutarate + O2
-
 Homo sapiens ? + succinate + CO2
-
 ?
(Pro-Pro-Gly)10 L-proline + 2-oxoglutarate + O2
-
 Homo sapiens (Pro-Pro-Gly)10 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
dansyl-Gly-Phe-Pro-Gly-OEt + 2-oxoglutarate + O2
-
 Homo sapiens dansyl-Gly-Phe-trans-4-hydroxy-L-proline-Gly-OEt + succinate + CO2
-
 ?
procollagen L-proline + 2-oxoglutarate + O2
-
 Homo sapiens procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2,beta2 Homo sapiens

Synonyms

Synonyms Comment Organism
P4H
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 dansyl-Gly-Phe-Pro-Gly-OEt
-
 Homo sapiens
0.22
-
 (Pro-Pro-Gly)10
-
 Homo sapiens