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Literature summary for 1.14.11.2 extracted from

  • Kivirikko, K.I.; Kishida, Y.; Sakakibara, S.; Prockop, D.J.
    Hydroxylation of (X-Pro-Gly)n by protocollagen proline hydroxylase. Effect of chain length, helical conformation and amino acid sequence in the substrate (1972), Biochim. Biophys. Acta, 271, 347-356.
    View publication on PubMed
Search for more literature for 1.14.11.2

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Gallus gallus
catalase activation Gallus gallus
dithiothreitol activation Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km decreases markedly with chain-length, it remains the same for the triple-helical conformation and for the random-coil form, and decreases by the presence of a basic amino acid in the NH2-terminal end Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 20 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 20 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 20 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 20 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
Arg-Gly-(Pro-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Gallus gallus 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
Glu-Gly-(Pro-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Gallus gallus 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Gallus gallus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate
-
 Gallus gallus
ascorbate
-
 Gallus gallus