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Literature summary for 1.14.11.2 extracted from

  • Tuderman, L.; Myllylä, R.; Kivirikko, K.I.
    Mechanism of the prolyl hydroxylase reaction. 1. Role of co-substrates (1977), Eur. J. Biochem., 80, 341-348.
    View publication on PubMed
Search for more literature for 1.14.11.2

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin activation Gallus gallus
catalase activation Gallus gallus
dithiothreitol activation Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
2-oxosuccinate i.e. oxaloacetate Gallus gallus
3-hydroxybutyrate less than 10% inhibition Gallus gallus
ascorbate at high concentrations Gallus gallus
citrate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
epinephrine competitive inhibition with respect to Fe2+ Gallus gallus
Fe2+ inhibitory at high concentrations Gallus gallus
fumarate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
isocitrate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
Lactate less than 10% inhibition Gallus gallus
malate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
nitroblue tetrazolium competitive inhibition with respect to O2 Gallus gallus
oxaloacetate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
poly(L-proline)
-
 Gallus gallus
pyruvate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
Zn2+ competitive inhibition with respect to Fe2+, non-competitive with respect to the polypeptide substrate and 2-oxoglutarate Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ absolute requirement, Km: 0.005 mM Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
procollagen L-proline + 2-oxoglutarate + O2 = procollagen trans-4-hydroxy-L-proline + succinate + CO2 mechanism, role of cosubstrates Gallus gallus
a

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
2-oxoglutarate + O2 + ascorbate uncoupled oxidative decarboxylation Gallus gallus succinate + dehydroascorbate + CO2 + H2O
-
 ?
additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Gallus gallus ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
ascorbate Km: 0.2 mM Gallus gallus
ascorbate requirement Gallus gallus
additional information the ascorbate requirement cannot be replaced by tetrahydrofolic acid, dithiothreitol, NADH2 or dithionite to any significant extent Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
 Zn2+
-
 Gallus gallus
0.04
-
 nitroblue tetrazolium
-
 Gallus gallus
0.1
-
 epinephrine
-
 Gallus gallus
0.4 0.5 citrate calculated from the slopes or intercepts Gallus gallus
0.5 0.6 fumarate calculated from the slopes or intercepts Gallus gallus
0.6
-
 Fe2+
-
 Gallus gallus
0.6
-
 oxaloacetate
-
 Gallus gallus
1.2 1.3 malate calculated from the slopes or intercepts Gallus gallus
1.5 2.1 isocitrate calculated from the slopes or intercepts Gallus gallus
7
-
 ascorbate
-
 Gallus gallus
15 18 pyruvate calculated from the slopes or intercepts Gallus gallus