Any feedback?
Literature summary for 1.14.11.2 extracted from
- Partial purification and characterization of prolyl hydroxylase from the free-living nematode Panagrellus silusiae (1978), Can. J. Zool., 56, 159-165.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| catalase | activation | Panagrellus silusiae | |
| dithiothreitol | activation | Panagrellus silusiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Panagrellus silusiae | |
| 2,2'-dipyridyl | - |
Panagrellus silusiae | |
| poly(L-proline) | - |
Panagrellus silusiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Panagrellus silusiae | |
| 0.005 | - |
Protocollagen | - |
Panagrellus silusiae | |
| 0.1 | 0.5 | carboxymethylated collagen L-proline | - |
Panagrellus silusiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Panagrellus silusiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
4 * 67000, SDS-PAGE | Panagrellus silusiae |
| 285000 | - |
gel filtration | Panagrellus silusiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Panagrellus silusiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using ammonium sulfate precipitation and calcium phosphate gel ion exchange | Panagrellus silusiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Panagrellus silusiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, half-life of ammonium sulfate precipitated enzyme: 7.3 days, stability of enzyme purified with calcium phosphate gel: 21 days, 20-30% loss of activity | Panagrellus silusiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylated collagen L-proline + 2-oxoglutarate + O2 | - |
Panagrellus silusiae | carboxymethylated collagen-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
| collagen + 2-oxoglutarate + O2 | - |
Panagrellus silusiae | 4-hydroxyproline containing collagen + succinate + CO2 | - |
? | |
| additional information | effect of substrate on activity | Panagrellus silusiae | ? | - |
? | |
| additional information | thermal denaturing of the triple-helical conformation of the substrate before hydroxylation | Panagrellus silusiae | ? | - |
? | |
| protocollagen + 2-oxoglutarate + O2 | - |
Panagrellus silusiae | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 67000, SDS-PAGE | Panagrellus silusiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Panagrellus silusiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Panagrellus silusiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Panagrellus silusiae | |
| ascorbate | - |
Panagrellus silusiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
