Activating Compound | Comment | Organism | Structure |
---|---|---|---|
catalase | activation | Panagrellus silusiae | |
dithiothreitol | activation | Panagrellus silusiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Panagrellus silusiae | |
2,2'-dipyridyl | - |
Panagrellus silusiae | |
poly(L-proline) | - |
Panagrellus silusiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Panagrellus silusiae | |
0.005 | - |
Protocollagen | - |
Panagrellus silusiae | |
0.1 | 0.5 | carboxymethylated collagen L-proline | - |
Panagrellus silusiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Panagrellus silusiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
4 * 67000, SDS-PAGE | Panagrellus silusiae |
285000 | - |
gel filtration | Panagrellus silusiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Panagrellus silusiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, using ammonium sulfate precipitation and calcium phosphate gel ion exchange | Panagrellus silusiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Panagrellus silusiae |
Storage Stability | Organism |
---|---|
-30°C, half-life of ammonium sulfate precipitated enzyme: 7.3 days, stability of enzyme purified with calcium phosphate gel: 21 days, 20-30% loss of activity | Panagrellus silusiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylated collagen L-proline + 2-oxoglutarate + O2 | - |
Panagrellus silusiae | carboxymethylated collagen-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
collagen + 2-oxoglutarate + O2 | - |
Panagrellus silusiae | 4-hydroxyproline containing collagen + succinate + CO2 | - |
? | |
additional information | effect of substrate on activity | Panagrellus silusiae | ? | - |
? | |
additional information | thermal denaturing of the triple-helical conformation of the substrate before hydroxylation | Panagrellus silusiae | ? | - |
? | |
protocollagen + 2-oxoglutarate + O2 | - |
Panagrellus silusiae | 4-hydroxyproline containing protocollagen + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 67000, SDS-PAGE | Panagrellus silusiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Panagrellus silusiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Panagrellus silusiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Panagrellus silusiae | |
ascorbate | - |
Panagrellus silusiae |