Literature summary for 1.14.11.17 extracted from

Henderson, K.L.; Mueller, T.A.; Hausinger, R.P.; Emerson, J.P.
Calorimetric assessment of Fe(2+) binding to ?-ketoglutarate/taurine dioxygenase ironing out the energetics of metal coordination by the 2-His-1-carboxylate facial triad (2015), Inorg. Chem., 54, 2278-2283 .

Search for more literature for 1.14.11.17

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli BL21(DE3) cells harboring plasmid pME4141	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the thermodynamic properties of Fe2+ binding to the 2-His-1-carboxylate facial triad in alpha-ketoglutarate/taurine dioxygenase (TauD) are explored using isothermal titration calorimetry. Direct titrations of Fe2+ into TauD and chelation experiments involving the titration of thylenediaminetetraacetic acid into Fe2+-TauD are performed under an anaerobic environment to yield a binding equilibrium of 2400000 (Kd = 43 nM) and a DELTAG(0) value of -10.1 kcal/mol. Further analysis of the enthalpy/entropy contributions indicates a highly enthalpic binding event, where DELTAH = -11.6 kcal/mol. Investigations into the unfavorable entropy term leads to the observation of water molecules becoming organized within the Fe2+-TauD structure	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P37610	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
taurine + 2-oxoglutarate + O2	-	Escherichia coli	sulfite + aminoacetaldehyde + succinate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
alpha-ketoglutarate/taurine dioxygenase	-	Escherichia coli
TauD	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Escherichia coli

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Release 2023.1 (January 2023)