Literature summary for 1.14.11.17 extracted from

Knauer, S.H.; Hartl-Spiegelhauer, O.; Schwarzinger, S.; Haenzelmann, P.; Dobbek, H.
The Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenases from Pseudomonas putida and Escherichia coli are tetramers (2012), FEBS J., 279, 816-831.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of TauD in Escherichia coli strains BL21(DE3) and Rosetta Blue(DE3)	Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment)	Organism
three crystal structures of the apo form, vapor diffusion techniques, protein solution contains 1. 27-28 m/ml TauDPp in 25 mM Tris-HCl, pH 7.7, or 2. 21 mg/ml TauDPp in 25 mM Tris-HCl pH 7.7, containing 10 mM taurine, 0.1 M (NH4)2SO4 and 20% v?v glycerol, mixed with reservoir solution containing 1. 15% w?v PEG 1000, 40% v?v PEG 400, 0.15 M NaK phosphate, and 0.1 M imidazole chloride, pH 6.5, or 2. 20% w?v PEG 5000 monomethyl ether, 0.1 M Bis-Tris-HCl, pH 6.5, X-ray diffraction structure determination and analysis at 1.85-2.6 A resolution	Pseudomonas putida

Crystallization (Comment)

Organism

three crystal structures of the apo form, vapor diffusion techniques, protein solution contains 1. 27-28 m/ml TauDPp in 25 mM Tris-HCl, pH 7.7, or 2. 21 mg/ml TauDPp in 25 mM Tris-HCl pH 7.7, containing 10 mM taurine, 0.1 M (NH4)2SO4 and 20% v?v glycerol, mixed with reservoir solution containing 1. 15% w?v PEG 1000, 40% v?v PEG 400, 0.15 M NaK phosphate, and 0.1 M imidazole chloride, pH 6.5, or 2. 20% w?v PEG 5000 monomethyl ether, 0.1 M Bis-Tris-HCl, pH 6.5, X-ray diffraction structure determination and analysis at 1.85-2.6 A resolution

Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	dependent on, mononuclear non-heme iron center, binding structure and kinetics, spectral analysis, overview	Escherichia coli
Fe2+	dependent on, mononuclear non-heme iron center, binding structure and kinetics, spectral analysis, overview	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	4 * 31000, about, sequence calculation, TauDPp is a tetramer in solution and in the crystals	Pseudomonas putida
32000	-	4 * 32000, about, sequence calculation, TauDEc is a tetramer in solution and in the crystals, gel filtration and sedimentation velocity analytical ultracentrifugation	Escherichia coli
110000	-	gel filtration	Escherichia coli
112000	-	gel filtration	Pseudomonas putida
120000	-	sedimentation velocity analytical ultracentrifugation	Pseudomonas putida
127000	-	sedimentation velocity analytical ultracentrifugation	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
taurine + 2-oxoglutarate + O2	Escherichia coli	-	sulfite + aminoacetaldehyde + succinate + CO2	-	?
taurine + 2-oxoglutarate + O2	Pseudomonas putida	-	sulfite + aminoacetaldehyde + succinate + CO2	-	?
taurine + 2-oxoglutarate + O2	Pseudomonas putida KT 2240	-	sulfite + aminoacetaldehyde + succinate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Pseudomonas putida	Q88RA3	-	-
Pseudomonas putida KT 2240	Q88RA3	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.56	-	purified enzyme, pH and temperature not specified in the publication	Escherichia coli
8.11	-	purified enzyme, pH and temperature not specified in the publication	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
taurine + 2-oxoglutarate + O2	-	Escherichia coli	sulfite + aminoacetaldehyde + succinate + CO2	-	?
taurine + 2-oxoglutarate + O2	-	Pseudomonas putida	sulfite + aminoacetaldehyde + succinate + CO2	-	?
taurine + 2-oxoglutarate + O2	-	Pseudomonas putida KT 2240	sulfite + aminoacetaldehyde + succinate + CO2	-	?

Subunits

Subunits	Comment	Organism
More	topology of an apo-TauDPp monomer,overview	Pseudomonas putida
tetramer	4 * 31000, about, sequence calculation, TauDPp is a tetramer in solution and in the crystals	Pseudomonas putida
tetramer	4 * 32000, about, sequence calculation, TauDEc is a tetramer in solution and in the crystals, gel filtration and sedimentation velocity analytical ultracentrifugation	Escherichia coli

Synonyms

Synonyms	Comment	Organism
Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenase	-	Escherichia coli
Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenase	-	Pseudomonas putida
TauD	-	Escherichia coli
TauD	-	Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	real-time NMR spectra to follow enzymatic turnover	Pseudomonas putida

General Information

General Information	Comment	Organism
additional information	metal-to-ligand charge-transfer transition involving the 2-oxoglutarate ligand with a six-coordinate metal center. Taurine does not coordinate the Fe(II) ion, but binds in its close vicinity, leading to loss of the coordinating water molecule. This change of the six-coordinate to five-coordinate Fe(II) center causes a small perturbation of the absorption spectrum	Escherichia coli
additional information	metal-to-ligand charge-transfer transition involving the 2-oxoglutarate ligand with a six-coordinate metal center. Taurine does not coordinate the Fe(II) ion, but binds in its close vicinity, leading to loss of the coordinating water molecule. This change of the six-coordinate to five-coordinate Fe(II) center causes a small perturbation of the absorption spectrum	Pseudomonas putida