Any feedback?
Literature summary for 1.14.11.17 extracted from
- Characterization of a sulfur-regulated oxygenative alkylsulfatase from Pseudomonas putida S-313 (2000), J. Biol. Chem., 275, 31661-31667.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | required for activity | Pseudomonas putida |  |
| ascorbate | 3fold increase of activity at 0.2 mM | Pseudomonas putida | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.1 mM EDTA abolishes activity completely | Pseudomonas putida | |
| additional information | no reduction in enzyme activity is observed for sulfate concentrations up to 10 mM | Pseudomonas putida | |
| nonyl sulfate | inhibits at concentrations between 1 and 10 mM | Pseudomonas putida | |
| SDS | inhibits at concentrations between 1 and 10 mM | Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required for activity, highest activity in the presence of 0.1 mM | Pseudomonas putida | |
| additional information | Ni2+, Co2+, Mn2+, Cu2+, Zn2+, Mg2+, and Ca2+ have no stimulatory effect | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30250 | - |
4 * 30250, gel filtration | Pseudomonas putida |
| 32000 | - |
4 * 32000, SDS-PAGE | Pseudomonas putida |
| 121000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida S-313 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Resource-Q anion-exchange column chromatography and Superdex 200 gel filtration | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hexyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida | hexanal + sulfite + succinate + CO2 | - |
? | |
| hexyl sulfate + 2-oxoglutarate + O2 | - |
Pseudomonas putida S-313 | hexanal + sulfite + succinate + CO2 | - |
? | |
| additional information | the AtsK enzyme is not involved in the utilization of taurine as a sulfur source | Pseudomonas putida | ? | - |
? | |
| additional information | the AtsK enzyme is not involved in the utilization of taurine as a sulfur source | Pseudomonas putida S-313 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 32000, SDS-PAGE | Pseudomonas putida |
| homotetramer | 4 * 30250, gel filtration | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-ketoglutarate-dependent dioxygenase | - |
Pseudomonas putida |
| AtsK | - |
Pseudomonas putida |
| oxygenative alkylsulfatase | - |
Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
in Tris-acetate buffer | Pseudomonas putida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
