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Literature summary for 1.14.11.15 extracted from

  • Kwak, S.S.; Kamiya, Y.; Sakurai, A.; Takahashi, N.; Graebe, J.
    Partial purification and characterization of gibberellin 3beta-hydroxylase from immature seeds of Phaseolus vulgaris (1988), Plant Cell Physiol., 29, 935-943.
No PubMed abstract available
Search for more literature for 1.14.11.15

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 2 mg/ml, 30% activation of purified enzyme, stimulation by bovine serum albumin and catalase is additive and less pronounced in crude cell extract Phaseolus vulgaris
catalase 0.5 mg/ml, 30% activation of purified enzyme, stimulation by catalase and bovine serum albumin is additive and less pronounced in crude cell extract Phaseolus vulgaris

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl
-
 Phaseolus vulgaris
Cd2+ 0.5 mM, strong Phaseolus vulgaris
Co2+ 0.5 mM, strong Phaseolus vulgaris
Cu2+ 0.5 mM, strong Phaseolus vulgaris
gibberellin a15 inhibits 3beta-hydroxylation of gibberellin a20 Phaseolus vulgaris
gibberellin a20
-
 Phaseolus vulgaris
gibberellin a44 inhibits 3beta-hydroxylation of gibberellin a20 Phaseolus vulgaris
gibberellin a5 inhibits 3beta-hydroxylation of gibberellin a20 Phaseolus vulgaris
gibberellin a9 inhibits 3beta-hydroxylation of gibberellin a20 Phaseolus vulgaris
Hg2+ 0.5 mM, strong Phaseolus vulgaris
Mn2+ 0.5 mM, strong Phaseolus vulgaris
Ni2+ 0.5 mM, strong Phaseolus vulgaris
Zn2+ 0.5 mM, strong Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00029
-
 gibberellin a20
-
 Phaseolus vulgaris
0.00033
-
 gibberellin a9
-
 Phaseolus vulgaris
0.25
-
 2-oxoglutarate with gibberellin a20 as cosubstrate Phaseolus vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activates, at least 0.2 mM for full activation Phaseolus vulgaris
Fe3+ activation in the presence of ascorbate Phaseolus vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 gel filtration Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
seed immature Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gibberellin a20 + 2-oxoglutarate + O2
-
 Phaseolus vulgaris gibberellin a1 + succinate + CO2
-
 ?
gibberellin a9 + 2-oxoglutarate + O2
-
 Phaseolus vulgaris gibberellin a4 + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Phaseolus vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about half-maximal activity at pH 7.0 and pH 9.0 Phaseolus vulgaris

Cofactor

Cofactor Comment Organism Structure
ascorbate 5 mM, 10fold stimulation Phaseolus vulgaris