Literature summary for 1.14.11.13 extracted from

Magome, H.; Yamaguchi, S.; Hanada, A.; Kamiya, Y.; Oda, K.
The DDF1 transcriptional activator upregulates expression of a gibberellin-deactivating gene, GA2ox7, under high-salinity stress in Arabidopsis (2008), Plant J., 56, 613-626.

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Cloned(Commentary)

Cloned (Comment)	Organism
reporter plasmid: promoter region of GA2ox7 amplified from genomic DNA by PCR, fused to luciferase gene in pUC19, effector plasmid: replacing GUS gene of pBI221 with DDF1 coding region, reference plasmid with Renilla luciferase gene, introduction of the plasmids to leaves via particle bombardment, leaves incubated on 0.8% agar plates at 22°C for 24 h. transgenic plants: PCR-amplificatoin of DDF1 coding region, cloned into pENTER-TOPO, inserted into vector pGWB8, Agrobacterium (strain EHA105)-mediated transformation of wild-type and GA2ox7-2 plants	Arabidopsis thaliana

Cloned (Comment)

Organism

reporter plasmid: promoter region of GA2ox7 amplified from genomic DNA by PCR, fused to luciferase gene in pUC19, effector plasmid: replacing GUS gene of pBI221 with DDF1 coding region, reference plasmid with Renilla luciferase gene, introduction of the plasmids to leaves via particle bombardment, leaves incubated on 0.8% agar plates at 22°C for 24 h. transgenic plants: PCR-amplificatoin of DDF1 coding region, cloned into pENTER-TOPO, inserted into vector pGWB8, Agrobacterium (strain EHA105)-mediated transformation of wild-type and GA2ox7-2 plants

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	loss-of-funtion mutation of GA2ox7 suppresses the dwarf phenotype of DWARF AND DELAYED FLOWERING 1 (DDF1) mutants, indicating that their gibberellin deficiency is due to overexpression of GA2ox7, DDF1 activates the promoter of GA2ox7 in leaves. Under high-salinity stress (250 mM), osmotic stress (300 mM mannitol) GA2ox7 is unregulated and slightly unregulated under the stress hormone abscisic acid (100 microM), the GA2ox7 loss-of-function mutant is less growth retarded than the wild-type under high-salinity stress	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
gibberellin + 2-oxoglutarate + O2	Arabidopsis thaliana	gibberellin 12 and 53	2beta-hydroxygibberellin + succinate + CO2	gibberellin 110, the product of gibberellin 12	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	wild-type Columbia (Col), DWARF AND DELAYED FLOWERING 1 (DDF1) overexpressing mutant, loss-of-funtion mutants ddf1-LF1 and ga20x7-2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
gibberellin + 2-oxoglutarate + O2	gibberellin 12 and 53	Arabidopsis thaliana	2beta-hydroxygibberellin + succinate + CO2	gibberellin 110, the product of gibberellin 12	?

Synonyms

Synonyms	Comment	Organism
C20-gibberellin deactivation enzyme	-	Arabidopsis thaliana
GA 2-oxidase 7	-	Arabidopsis thaliana
GA2ox7	-	Arabidopsis thaliana
gibberellin 2-oxidase	-	Arabidopsis thaliana