Cloned (Comment) | Organism |
---|---|
full-length coding sequence of GA2ox6 amplified by PCR, cloned into pET32a to be expressed as fusion with thioredoxin in Escherichia coli BL21(DE3) for in vitro enzyme activity measurements | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | single loss-of-function mutants do not exhibit any obvious phenotype, quintuple mutant (loss of function alleles of all five C19-gibberellin 2-oxidase genes) leads to a complete loss of C19-gibberellin 2-oxidase (C19-GA2ox) activity, with effects on other gibberellin regulated genes (reduced expression of gibberellin biosynthetic genes) and positive regulation of the C20-GA2ox gene GA20x8, like the positive regulation of 4 of the 5 C19-GA2ox genes with the exception of GA2ox3, thus, partial compensation for the activity loss of C19-GA2ox is reached by a combination of feedback and feed-forward regulation of various steps in the gibberellin biosynthesis pathway | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin + 2-oxoglutarate + O2 + ? | Arabidopsis thaliana | - |
2beta-hydroxygibberellin + succinate + CO2 | inactivation of the plant hormone gibberellin by oxidation | ? | |
additional information | Arabidopsis thaliana | 5 different gibberellin 2-oxidases regulate plant development at various stages by prevention of seed germination in the absence of light and cold stimuli, delay of vegetative and floral phase transition, limitation of the number of flowers produced per inflorescence, suppression of elongation of the pistil prior to fertilization, limitation of main stem and side shoot elongation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O64692 | GA2ox3; wild-type Col-0, Ws-0, Ws-4 | - |
Arabidopsis thaliana | Q8LEA2 | GA2ox1; wild-type Col-0, Ws-0, Ws-4 | - |
Arabidopsis thaliana | Q9C7Z1 | GA2ox4; wild-type Col-0, Ws-0, Ws-4 | - |
Arabidopsis thaliana | Q9FZ21 | GA2ox6; wild-type Col-0, Ws-0, Ws-4 | - |
Arabidopsis thaliana | Q9XFR9 | GA2ox2; wild-type Col-0, Ws-0, Ws-4 | - |
Purification (Comment) | Organism |
---|---|
24 h after application of 14C-labelled substrate to the shoot apex, whole shoots are harvested, rinsed with water, same genotype shoots combined and homogenized in 80% methanol water, stirring overnight at 4°C, filtration, residue re-extracted in methanol for 1 h, filtered, combined filtrates vacuum dried, weak acid fraction prepared by solvent partitioning, anion exchange, and C18 SPE (no solvent partitioning for substrate gibberellin 9), purified samples are analyzed by reverse-phase HPLC with online radiomonitoring, identification of radiolabeled products by GC-MS | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | 24 d inflorescence: expression of GA2ox1, GA2ox2 (dominant), GA2ox3, GA2ox4, GA2ox6 (second highest level) | Arabidopsis thaliana | - |
leaf | 24 d: expression of GA2ox1, GA2ox3, GA2ox2 and GA2ox6 dominant, no GA2ox4 expression | Arabidopsis thaliana | - |
root | 7 d: expression of GA2ox1, GA2ox2, GA2ox3, GA2ox4, GA2ox6 dominant | Arabidopsis thaliana | - |
rosette | 11 d, expression of GA2ox1, GA2ox2 (dominant), GA2ox3, GA2ox4, GA2ox6 (second highest level) | Arabidopsis thaliana | - |
seed | dry seed: GA2ox1 and GA2ox4 not expressed, high levels of GA2ox2 and GA2ox3, GA2ox6 dominant, 24 h inhibited seed: low expression of GA2ox1 and GA2ox4, highest expression of GA2ox2 and GA2ox6, expression of GA2ox3 | Arabidopsis thaliana | - |
seedling | 3 d: expression of GA2ox1, GA2ox2 (second highest level), GA2ox3, GA2ox4, and GA2ox6 (dominant) | Arabidopsis thaliana | - |
shoot | 7 d: expression of GA2ox1, GA2ox3 (very low expression), GA2ox4, GA2ox2 and GA2ox6 dominant | Arabidopsis thaliana | - |
silique | 31 d: expression of GA2ox1, GA2ox2 (dominant), GA2ox3, GA2ox4, GA2ox6 (second highest level) | Arabidopsis thaliana | - |
stem | 24 d: expression of GA20x1, GA2ox2 (dominant), GA2ox3, GA2ox6 (second highest level), no GA2ox4 expression | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin + 2-oxoglutarate + O2 + ? | - |
Arabidopsis thaliana | 2beta-hydroxygibberellin + succinate + CO2 | inactivation of the plant hormone gibberellin by oxidation | ? | |
gibberellin 1 + 2-oxoglutarate + O2 | GA2ox6 in vitro activity | Arabidopsis thaliana | gibberellin 8 + succinate + CO2 | - |
? | |
gibberellin 20 + 2-oxoglutarate + O2 | GA2ox6 in vitro activity | Arabidopsis thaliana | gibberellin 29 + succinate + CO2 | - |
? | |
gibberellin 3 + 2-oxoglutarate + O2 | measurement of gibberellin responsiveness of GA2ox genes in the developmental series of wild-type plants, spraying of the plants with 100 microM substrate | Arabidopsis thaliana | ? | - |
? | |
gibberellin 4 + 2-oxoglutarate + O2 | GA2ox6 in vitro activity and other gibberellin 2-oxidase activities with 14C-labeled substrate (in 10% methanol) applied to the shoot apex of plants | Arabidopsis thaliana | gibberellin 34 + succinate + CO2 | - |
? | |
gibberellin 9 + 2-oxoglutarate + O2 | GA2ox6 in vitro activity and other gibberellin 2-oxidase activities with 14C-labeled substrate (in 10% methanol) applied to the shoot apex of plants | Arabidopsis thaliana | gibberellin 51 + succinate + CO2 | - |
? | |
additional information | 5 different gibberellin 2-oxidases regulate plant development at various stages by prevention of seed germination in the absence of light and cold stimuli, delay of vegetative and floral phase transition, limitation of the number of flowers produced per inflorescence, suppression of elongation of the pistil prior to fertilization, limitation of main stem and side shoot elongation | Arabidopsis thaliana | ? | - |
? | |
additional information | no products detectable of GA2ox6 (in vitro activity) with C20-gibberellin substrates gibberellin 12 and 53 and with C19-gibberellin substrate 7 | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C19-GA 2-oxidase | - |
Arabidopsis thaliana |
GA2ox | - |
Arabidopsis thaliana |
GA2ox1 | class I, acts as C19-GA 2-oxidase | Arabidopsis thaliana |
GA2ox2 | class I, acts as C19-GA 2-oxidase, most highly expressed throughout development | Arabidopsis thaliana |
GA2ox3 | class I, acts as C19-GA 2-oxidase | Arabidopsis thaliana |
GA2ox4 | class II, acts exclusively as C19-GA 2-oxidase | Arabidopsis thaliana |
GA2ox6 | class II, acts as C19-GA 2-oxidase, most highly expressed throughout development, is reported to have activity against GA4 and GA1, in this study no C20-GA 2-oxidase activity against GA12 and GA53 in an in vitro assay with lysates of Escherichia coli expressing GA2ox6 and 14C-labeled gibberellin substrates | Arabidopsis thaliana |
gibberellin 2-oxidase | - |
Arabidopsis thaliana |