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Literature summary for 1.14.11.13 extracted from

  • Smith, V.A.; MacMillan, J.
    Purification and partial characterization of a gibberellin 2beta-hydroxylase from Phaseolus vulgaris (1984), J. Plant Growth Regul., 2, 251-264.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
catalase activity is stimulated by Phaseolus vulgaris

General Stability

General Stability Organism
dialysis of the extracted soluble protein results in complete loss of activity Phaseolus vulgaris

Inhibitors

Inhibitors Comment Organism Structure
Gibberellin methyl ester
-
Phaseolus vulgaris
N,N-dimethyl succinic acid hydrazide
-
Phaseolus vulgaris
succinic acid
-
Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000085
-
[1,2-3H2]gibberellin 1
-
Phaseolus vulgaris
0.021
-
alpha-ketoglutarate
-
Phaseolus vulgaris

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Phaseolus vulgaris
Fe2+ required as cofactor, stimulated by addition of exogenous Fe2+ Phaseolus vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
SDS-PAGE Phaseolus vulgaris
36000
-
gel filtration Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
gibberellin 1 + 2-oxoglutarate + O2 Phaseolus vulgaris
-
gibberellin 8 + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
var. Canadian Wonder
-

Purification (Commentary)

Purification (Comment) Organism
-
Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Phaseolus vulgaris
-
seed
-
Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
0.000000000191mol per h Phaseolus vulgaris

Storage Stability

Storage Stability Organism
-20°C, enzyme activity lost upon storage, regained by addition of catalase to the reaction mixture Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gibberellin 1 + 2-oxoglutarate + O2 marked preference for 3-hydroxylated gibberellins as substrate Phaseolus vulgaris 2beta-hydroxygibberellin 1 + succinate + CO2
-
?
gibberellin 1 + 2-oxoglutarate + O2
-
Phaseolus vulgaris gibberellin 8 + succinate + CO2
-
?
gibberellin 20 + 2-oxoglutarate + O2
-
Phaseolus vulgaris gibberellin 29 + succinate + CO2
-
?
gibberellin 4 + 2-oxoglutarate + O2
-
Phaseolus vulgaris gibberellin 34 + succinate + CO2
-
?
gibberellin 9 + 2-oxoglutarate + O2
-
Phaseolus vulgaris gibberellin 51 + succinate + CO2
-
?
[1,2-3H2]gibberellin 1 + 2-oxoglutarate + O2
-
Phaseolus vulgaris ?
-
?

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate
-
Phaseolus vulgaris
ascorbate
-
Phaseolus vulgaris