Activating Compound | Comment | Organism | Structure |
---|---|---|---|
catalase | activity is stimulated by | Phaseolus vulgaris |
General Stability | Organism |
---|---|
dialysis of the extracted soluble protein results in complete loss of activity | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Gibberellin methyl ester | - |
Phaseolus vulgaris | |
N,N-dimethyl succinic acid hydrazide | - |
Phaseolus vulgaris | |
succinic acid | - |
Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000085 | - |
[1,2-3H2]gibberellin 1 | - |
Phaseolus vulgaris | |
0.021 | - |
alpha-ketoglutarate | - |
Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Phaseolus vulgaris | |
Fe2+ | required as cofactor, stimulated by addition of exogenous Fe2+ | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Phaseolus vulgaris |
36000 | - |
gel filtration | Phaseolus vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin 1 + 2-oxoglutarate + O2 | Phaseolus vulgaris | - |
gibberellin 8 + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
var. Canadian Wonder | - |
Purification (Comment) | Organism |
---|---|
- |
Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Phaseolus vulgaris | - |
seed | - |
Phaseolus vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.000000000191mol per h | Phaseolus vulgaris |
Storage Stability | Organism |
---|---|
-20°C, enzyme activity lost upon storage, regained by addition of catalase to the reaction mixture | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin 1 + 2-oxoglutarate + O2 | marked preference for 3-hydroxylated gibberellins as substrate | Phaseolus vulgaris | 2beta-hydroxygibberellin 1 + succinate + CO2 | - |
? | |
gibberellin 1 + 2-oxoglutarate + O2 | - |
Phaseolus vulgaris | gibberellin 8 + succinate + CO2 | - |
? | |
gibberellin 20 + 2-oxoglutarate + O2 | - |
Phaseolus vulgaris | gibberellin 29 + succinate + CO2 | - |
? | |
gibberellin 4 + 2-oxoglutarate + O2 | - |
Phaseolus vulgaris | gibberellin 34 + succinate + CO2 | - |
? | |
gibberellin 9 + 2-oxoglutarate + O2 | - |
Phaseolus vulgaris | gibberellin 51 + succinate + CO2 | - |
? | |
[1,2-3H2]gibberellin 1 + 2-oxoglutarate + O2 | - |
Phaseolus vulgaris | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Phaseolus vulgaris | |
ascorbate | - |
Phaseolus vulgaris |