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Literature summary for 1.14.11.11 extracted from
- Molecular cloning, expression and characterization of hyoscyamine 6beta-hydroxylase from hairy roots of Anisodus tanguticus (2005), Planta Med., 71, 249-253.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Anisodus tanguticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, complete inhibition | Anisodus tanguticus |  |
| Cu2+ | 5 mM, complete inhibition | Anisodus tanguticus | |
| EDTA | 5 mM, 61% inhibition | Anisodus tanguticus | |
| Fe3+ | 5 mM, 65% inhibition | Anisodus tanguticus | |
| Mg2+ | 5 mM, 14% inhibition | Anisodus tanguticus | |
| Mn2+ | 5 mM, complete inhibition | Anisodus tanguticus | |
| Ni2+ | 5 mM, 64% inhibition | Anisodus tanguticus | |
| Zn2+ | 5 mM, 66% inhibition | Anisodus tanguticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0151 | - |
hyoscyamine | pH 7.4 | Anisodus tanguticus | |
| 0.017 | - |
(6S)-hydroxyhyoscyamine | pH 7.4 | Anisodus tanguticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | without addition of Fe2+, only 1.3% of the activity is detected | Anisodus tanguticus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | Anisodus tanguticus | key enzyme in the biosynthesis pathway of scopolamine | scopolamine + succinate + CO2 | - |
? | |
| L-hyoscyamine + 2-oxoglutarate + O2 | Anisodus tanguticus | key enzyme in the biosynthesis pathway of scopolamine | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anisodus tanguticus | Q6V0J1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Anisodus tanguticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | hairy root | Anisodus tanguticus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Anisodus tanguticus | scopolamine + succinate + CO2 | - |
? | |
| (6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | key enzyme in the biosynthesis pathway of scopolamine | Anisodus tanguticus | scopolamine + succinate + CO2 | - |
? | |
| (6S)-hydroxyhyoscyamine + succinate + CO2 | - |
Anisodus tanguticus | L-hyoscyamine + 2-oxoglutarate + O2 | - |
r | |
| L-hyoscyamine + 2-oxoglutarate + O2 | - |
Anisodus tanguticus | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
| L-hyoscyamine + 2-oxoglutarate + O2 | key enzyme in the biosynthesis pathway of scopolamine | Anisodus tanguticus | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtH6H | - |
Anisodus tanguticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Anisodus tanguticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Anisodus tanguticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.4 | pH 5.0: 20% of maximal activity, pH 7.4: optimum | Anisodus tanguticus |
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