Application | Comment | Organism |
---|---|---|
medicine | inhibition of BBOX is reported to aid in recovery after acute stroke | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzeneselenenic acid | - |
Homo sapiens | |
benzeneselenenyl bromide | - |
Homo sapiens | |
benzeneselenenyl chloride | - |
Homo sapiens | |
benzeneseleninic acid | - |
Homo sapiens | |
diphenyldiselane | - |
Homo sapiens | |
ebselen | a relatively potent BBOX inhibitor and a Zn(II) ejector | Homo sapiens | |
N-oxalylglycine | a 2-oxoglutarate mimic | Homo sapiens | |
N-[(1-chloro-4-hydroxy-3-isoquinolinyl)carbonyl]-glycine | i.e. FG-2216, BIQ, or IOX3, an isoquinoline derivative, that inhibits BBOX and other 2-oxoglutarate dependent oxygenases through chelating active site Fe(II) and interacting with specific active site residues. Time course of Zn(II) ejection from BBOX by the inhibitors | Homo sapiens | |
thiram | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on, required for catalysis, bound to the active site | Homo sapiens | |
Zn2+ | dependent on, ejection of structural zinc, e.g. by Selenium and sulfur based compounds, leads to inhibition of the enzyme, an undisrupted N-terminal zinc binding domain of BBOX is required for catalysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-trimethylammoniobutanoate + 2-oxoglutarate + O2 | Homo sapiens | - |
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75936 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-trimethylammoniobutanoate + 2-oxoglutarate + O2 | - |
Homo sapiens | 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | BBOX is a homodimer comprising of catalytic domain where Fe(II) and substrates bind and zinc binding domain | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
BBOX | - |
Homo sapiens |
gamma-butyrobetaine hydroxylase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00083 | - |
pH 7.0, 22°C, 20 min | Homo sapiens | ebselen | |
0.0029 | - |
pH 7.0, 22°C, 20 min | Homo sapiens | benzeneselenenyl chloride | |
0.003 | - |
pH 7.0, 22°C, 20 min | Homo sapiens | benzeneselenenyl bromide | |
0.0047 | - |
pH 7.0, 22°C, 20 min | Homo sapiens | benzeneseleninic acid | |
0.0079 | - |
pH 7.0, 22°C, 20 min | Homo sapiens | thiram | |
0.019 | - |
pH 7.0, 22°C, 20 min | Homo sapiens | benzeneselenenic acid | |
0.032 | - |
pH 7.0, 22°C, 20 min | Homo sapiens | N-[(1-chloro-4-hydroxy-3-isoquinolinyl)carbonyl]-glycine | |
0.042 | - |
pH 7.0, 22°C, 20 min | Homo sapiens | diphenyldiselane |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the 2-oxoglutarate/Fe(II) dependent oxygenase family | Homo sapiens |
metabolism | the enzyme catalyses the second, essential step of carnitine biosynthesis | Homo sapiens |