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Literature summary for 1.14.11.1 extracted from

  • Kondo, A.; Blanchard, J.S.; Englard, S.
    Purification and properties of calf liver gamma-butyrobetaine hydroxylase (1981), Arch. Biochem. Biophys., 212, 338-346.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
catalase
-
Bos taurus

General Stability

General Stability Organism
dialysis, 4°C, 50% loss of activity overnight Bos taurus
ultra-filtration, 4°C, 50% loss of activity overnight Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
alpha,alpha'-bipyridyl no activity at 2 mM Bos taurus
Ba2+
-
Bos taurus
Ca2+
-
Bos taurus
Cd2+
-
Bos taurus
Co2+
-
Bos taurus
Cu2+
-
Bos taurus
dioxane
-
Bos taurus
Hg2+
-
Bos taurus
Mg2+
-
Bos taurus
Mn2+
-
Bos taurus
Ni2+
-
Bos taurus
Pb2+
-
Bos taurus
Sn2+
-
Bos taurus
Zn2+
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Bos taurus
0.012
-
Fe2+
-
Bos taurus
0.51
-
gamma-butyrobetaine
-
Bos taurus
0.82
-
2-oxoglutarate
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Bos taurus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ requirement Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
2 * 42000, SDS-PAGE Bos taurus
46000
-
2 * 46000, SDS-PAGE Bos taurus
80000
-
gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-trimethylammoniobutanoate + 2-oxoglutarate + O2 Bos taurus gamma-butyrobetaine, terminal reaction in the pathway of L-carnitine biosynthesis 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone powder, retains full activity of fresh liver homogenate Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.053
-
-
Bos taurus

Storage Stability

Storage Stability Organism
-90°C, stable for a year or more as acetone powder, stable for 3 months as purified and lyophilized enzyme Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
Bos taurus 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
?
4-trimethylammoniobutanoate + 2-oxoglutarate + O2 gamma-butyrobetaine, terminal reaction in the pathway of L-carnitine biosynthesis Bos taurus 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Bos taurus
dimer 2 * 46000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
ascorbate
-
Bos taurus