Cloned (Comment) | Organism |
---|---|
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX1, quantitative real-time PCR enzyme expression analysis | Arabidopsis thaliana |
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX2, quantitative real-time PCR enzyme expression analysis | Arabidopsis thaliana |
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX4, quantitative real-time PCR enzyme expression analysis | Arabidopsis thaliana |
in Arabidpsis thaliana, myo-inositol oxygenase is encoded by four genes, gene MIOX5, quantitative real-time PCR enzyme expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a quadruple (miox1/2/4/5) mutant by mutation of all four MIOX isozymes via T-DNA insertion, metabolite profiling of syncytia induced in roots of the myo-inositol oxygenase quadruple mutant, overview. The quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. The miox1/2/4/5 plants show a similar reduction in the number of females to miox double mutants | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + O2 | Arabidopsis thaliana | - |
D-glucuronate + H2O | - |
? | |
myo-inositol + O2 | Arabidopsis thaliana Col-0 | - |
D-glucuronate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O82200 | - |
- |
Arabidopsis thaliana | Q8H1S0 | - |
- |
Arabidopsis thaliana | Q8L799 | - |
- |
Arabidopsis thaliana | Q9FJU4 | - |
- |
Arabidopsis thaliana Col-0 | O82200 | - |
- |
Arabidopsis thaliana Col-0 | Q8H1S0 | - |
- |
Arabidopsis thaliana Col-0 | Q8L799 | - |
- |
Arabidopsis thaliana Col-0 | Q9FJU4 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pollen | MIOX4 is highly expressed in pollen | Arabidopsis thaliana | - |
pollen | MIOX5 is highly expressed in pollen | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
seedling | MIOX1 is expressed preferentially in seedlings | Arabidopsis thaliana | - |
seedling | MIOX2 is expressed preferentially in seedlings | Arabidopsis thaliana | - |
syncytium | high MIOX1 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview | Arabidopsis thaliana | - |
syncytium | high MIOX2 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview | Arabidopsis thaliana | - |
syncytium | high MIOX4 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview | Arabidopsis thaliana | - |
syncytium | high MIOX5 expression level in syncytia, induced in roots by attack through the beet cyst nematode Heterodera schachtii, metabolome analysis of syncytium, overview | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + O2 | - |
Arabidopsis thaliana | D-glucuronate + H2O | - |
? | |
myo-inositol + O2 | - |
Arabidopsis thaliana Col-0 | D-glucuronate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MIOX | - |
Arabidopsis thaliana |
MIOX1 | - |
Arabidopsis thaliana |
MIOX2 | - |
Arabidopsis thaliana |
MIOX4 | - |
Arabidopsis thaliana |
MIOX5 | - |
Arabidopsis thaliana |
Myo-inositol oxygenase | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | all MIOX genes are strongly expressed in syncytia induced by the beet cyst nematode Heterodera schachtii in Arabidopsis thaliana roots | up |
General Information | Comment | Organism |
---|---|---|
malfunction | the quadruple myo-inositol oxygenase mutant shows a significant reduction in susceptibility to Heterodera schachtii, and syncytia have elevated myo-inositol and galactinol levels and an elevated expression level of the antimicrobial thionin gene Thi2.1. This reduction in susceptibility can also be achieved by exogenous application of galactinol to wild-type seedlings. Except for MIOX2, transcripts for the other three MIOX genes are still detected at a level of 2-14% of their abundance in the wild-type, the mutant does not show any visible phenotype and produced viable pollen, but the incorporation of myo-inositol-derived sugars into cell walls is strongly inhibited by over 90% with no change in the ultrastructure of syncytial cell walls | Arabidopsis thaliana |
physiological function | the enzyme myo-inositol oxygenase is the key enzyme of a pathway leading from myo-inositol to UDP-glucuronic acid. The primary function of myo-inositol oxygenase for syncytium development after infection with Heterodera schachtii is probably not the production of UDP-glucuronic acid as a precursor for cell wall polysaccharides, but the reduction of myo-inositol levels and thereby a reduction in the galactinol level to avoid the induction of defence-related genes | Arabidopsis thaliana |