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Literature summary for 1.13.99.1 extracted from
- Production of glucaric acid from myo-inositol in engineered Pichia pastoris (2016), Enzyme Microb. Technol., 91, 8-16 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene miox, recombinant expression in Pichia pastoris, coexpression with urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 as fusion protein | Komagataella phaffii |
| gene mMIOX, recombinant expression in Pichia pastoris, coexpression with urinate dehydrogenase (Udh) from Pseudomonas putida KT2440, recombinant coexpression of the enzyme in Escherichia coli with myo-inositol-1-phosphate synthase from Saccharomyces cerevisiae and urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | coexpression of the Mus musculus mMIOX and the urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 as fusion protein leads highly increased glutaric acid levels from myo-inositol in transfected Pichia pastoris cells. Production of glucaric acid with a fed-batch fermentation strategy, overview. Recombinant coexpression of the enzyme in Escherichia coli strains BL21(DE3) or MG1655 with myo-inositol-1-phosphate synthase from Saccharomyces cerevisiae and urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 leads to highly increased glutaric acid production, especially with addition of myo-inositol instead of glucose, production parameters, overview | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol + O2 | Mus musculus | - |
D-glucuronate + H2O | - |
? |  |
| myo-inositol + O2 | Komagataella phaffii | - |
D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | Komagataella phaffii GS115 | - |
D-glucuronate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Komagataella phaffii | C4QZH8 | i.e. Pichia pastoris | - |
| Komagataella phaffii GS115 | C4QZH8 | i.e. Pichia pastoris | - |
| Mus musculus | Q9QXN5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol + O2 | - |
Mus musculus | D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | - |
Komagataella phaffii | D-glucuronate + H2O | - |
? | |
| myo-inositol + O2 | - |
Komagataella phaffii GS115 | D-glucuronate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MIOX | - |
Mus musculus |
| MIOX | - |
Komagataella phaffii |
| mMIOX | - |
Mus musculus |
| Myo-inositol oxygenase | - |
Mus musculus |
| Myo-inositol oxygenase | - |
Komagataella phaffii |
| ppMIOX | - |
Komagataella phaffii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Komagataella phaffii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Mus musculus |
| 8 | - |
assay at | Komagataella phaffii |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Komagataella phaffii | coexpression of the Pichia pastoris ppMIOX and the urinate dehydrogenase (Udh) from Pseudomonas putida KT2440 does not lead to accumulation of glucaric acid from myo-inositol or increased enzyme activity of neither MIOX nor Udh | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is important in the glucaric acid synthetic pathway | Mus musculus |
| metabolism | the enzyme is important in the glucaric acid synthetic pathway | Komagataella phaffii |
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