Literature summary for 1.13.12.6 extracted from

Wu, C.; Irie, S.; Yamamoto, S.; Ohmiya, Y.
A bioluminescent enzyme immunoassay for prostaglandin E(2) using Cypridina luciferase (2009), Luminescence, 24, 131-133.

Search for more literature for 1.13.12.6

Application

Application	Comment	Organism
diagnostics	labeling of prostaglandin E2 with the bioluminescent enzyme Cypridina luciferase linked to monoclonal anti-prostaglandin E2 antibody as enzyme-linked immunosorbent assay (ELISA) system, detection of 7.8-500 pg/ml prostaglandin E2	Cypridina noctiluca

Protein Variants

Protein Variants	Comment	Organism
additional information	conjugation of prostaglandin E2 to luciferase by producing the N-hydroxysuccinimidyl ester of prostaglandin E2 by reaction of N-hydroxysuccinimde and 1-ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride and conjugation of the ester to luciferase (ratio 18/1) in 0.1 M potassium phosphate buffer, pH 7.2, with 0.15 M NaCl for 13 h on ice	Cypridina noctiluca

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0016	-	Cypridina luciferin	35 nM recombinant prostaglandin E2-luciferase diluted with 0.1 M Tris buffer (pH 7.4) containing 0.05% bovine serum albumin, 1 nM monoclonal anti-prostaglandin E2 antibody, incubation for 18 h, 4°C, in the dark, addition of 1 microM Cypridina luciferin in 0.1 M Tris-HCl buffer (pH 7.4) containing 0.3 M sodium ascorbate and 0.02 M Na2SO3 for bioluminescence reading	Cypridina noctiluca

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Cypridina luciferin + O2	Cypridina noctiluca	-	oxidized Cypridina luciferin + CO2 + hv	bioluminescence	?

Organism

Organism	UniProt	Comment	Textmining
Cypridina noctiluca	-	sea-firefly	-

Purification (Commentary)

Purification (Comment)	Organism
loading of recombinant prostaglandin E2 luciferase onto size-exclusion column, elution with 0.1 M potassium phosphate buffer, pH 7.2, and 0.15 M NaCl, fractions with bioluminescent activities (Cypridina luciferin solution, 0.1 M Tris-HCl, pH 7.4, 0.3 M sodium ascorbate, 0.2 M Na2SO3) are combined and stored at -30°C	Cypridina noctiluca

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	recombinant luciferase exhibits 14% activity of wild-type Cypridina luciferase	Cypridina noctiluca

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Cypridina luciferin + O2	-	Cypridina noctiluca	oxidized Cypridina luciferin + CO2 + hv	bioluminescence	?

Synonyms

Synonyms	Comment	Organism
Cypridina luciferase	-	Cypridina noctiluca
PGE2-luciferase	recombinant luciferase conjugated with N-hydroxysuccinimidyl ester of prostaglandin E2	Cypridina noctiluca

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Release 2023.1 (January 2023)