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Literature summary for 1.13.12.5 extracted from

  • Jiang, T.; Yang, X.; Yang, X.; Yuan, M.; Zhang, T.; Zhang, H.; Li, M.
    Novel bioluminescent coelenterazine derivatives with imidazopyrazinone C-6 extended substitution for Renilla luciferase (2016), Org. Biomol. Chem., 14, 5272-5281 .
    View publication on PubMed
Search for more literature for 1.13.12.5

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in ES-2 cells Renilla reniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Renilla reniformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coelenterazine + O2 Renilla reniformis
-
 coelenteramide + CO2 + hv
-
 ?

Organism

Organism UniProt Comment Textmining
Renilla reniformis P27652
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-benzyl-8-benzyl-6-(2-fluorophenylethynyl)imidazo[1,2-a]pyrazin-3(7H)-one + O2
-
 Renilla reniformis ? + CO2 + hv
-
 ?
2-benzyl-8-benzyl-6-(3-fluorophenylethynyl)imidazo[1,2-a]pyrazin-3(7H)-one + O2
-
 Renilla reniformis ? + CO2 + hv
-
 ?
2-benzyl-8-benzyl-6-(3-hydroxyphenylethynyl)imidazo[1,2-a]pyrazin-3(7H)-one + O2
-
 Renilla reniformis ? + CO2 + hv
-
 ?
2-benzyl-8-benzyl-6-(3-methylphenylethynyl)imidazo[1,2-a]pyrazin-3(7H)-one + O2
-
 Renilla reniformis ? + CO2 + hv
-
 ?
2-benzyl-8-benzyl-6-(4-fluorophenylethynyl)imidazo[1,2-a]pyrazin-3(7H)-one + O2
-
 Renilla reniformis ? + CO2 + hv
-
 ?
2-benzyl-8-benzyl-6-(phenylethynyl)imidazo[1,2-a]pyrazin-3(7H)-one + O2
-
 Renilla reniformis ? + CO2 + hv
-
 ?
2-benzyl-8-benzyl-6-[(1-fluoroethyl)-1,2,3-triazol-4]imidazo[1,2-a]pyrazin-3(7H)-one + O2
-
 Renilla reniformis ? + CO2 + hv
-
 ?
2-benzyl-8-benzyl-6-[(1-hydroxyethyl)-1,2,3-triazol-4]imidazo[1,2-a]pyrazin-3(7H)-one + O2
-
 Renilla reniformis ? + CO2 + hv
-
 ?
2-benzyl-8-benzyl-6-[(1-hydroxypropyl)-1,2,3-triazol-4]imidazo[1,2-a]pyrazin-3(7H)-one + O2
-
 Renilla reniformis ? + CO2 + hv
-
 ?
coelenterazine + O2
-
 Renilla reniformis coelenteramide + CO2 + hv
-
 ?
additional information design and synthesis of bioluminescent coelenterazine derivatives (alkynes and triazoles) with imidazopyrazinone C-6 extended substitution as substrates for Renilla luciferase, evaluation, substrate specificity, overview Renilla reniformis ?
-
 ?

Synonyms

Synonyms Comment Organism
Renilla luciferase
-
 Renilla reniformis
RLuc
-
 Renilla reniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Renilla reniformis