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Literature summary for 1.13.12.5 extracted from

  • Herbst, K.J.; Allen, M.D.; Zhang, J.
    The cAMP-dependent protein kinase inhibitor H-89 attenuates the bioluminescence signal produced by renilla luciferase (2009), Plos One, 4, e5642.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.12.5

Application

Application Comment Organism
pharmacology investigations into regulation and functional roles of kinases Renilla sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells Renilla sp.
expression of RLuc8 in Escherichia coli BL21 Renilla sp.

Protein Variants

Protein Variants Comment Organism
RLuc8 eight-point mutation variant of renilla luciferase Renilla sp.

Inhibitors

Inhibitors Comment Organism Structure
H-89 10 microM, reduced luminescence to 50% of RLuc and to 54% of RLuc8 Renilla sp.

Organism

Organism UniProt Comment Textmining
Renilla sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Renilla sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
b-coelenterazine + O2
-
 Renilla sp. ?
-
 ?
D-luciferin + O2
-
 Renilla sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
firefly luciferase
-
 Renilla sp.
fluc
-
 Renilla sp.
Renilla luciferase
-
 Renilla sp.
RLuc
-
 Renilla sp.
RLuc8 eight-point mutation variant of RLuc Renilla sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.021
-
 10 microM coelenterazine, measured RLuc8 activity Renilla sp. H-89