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Literature summary for 1.13.12.5 extracted from

  • Suzuki, T.; Usuda, S.; Ichinose, H.; Inouye, S.
    Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase (2007), FEBS Lett., 581, 4551-4556.
    View publication on PubMed
Search for more literature for 1.13.12.5

Application

Application Comment Organism
additional information visualize proteins being secreted by exocytosis Gaussia princeps

Cloned(Commentary)

Cloned (Comment) Organism
expression in CHO-K1 cells and PC12D cells Gaussia princeps

Protein Variants

Protein Variants Comment Organism
DBHsp-GLuc dopamine beta-hydroxlase fused to GLase, lower luminescence activity than wild-type GLuc Gaussia princeps
deltaSP-GLuc GLuc without signal peptide sequence, no significant luminescence Gaussia princeps

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol reducing agent Gaussia princeps
dithiothreitol reducing agent Gaussia princeps

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Gaussia princeps 5886
-
secretory vesicle
-
 Gaussia princeps 99503
-

Organism

Organism UniProt Comment Textmining
Gaussia princeps
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gaussia princeps

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coelenterazine + O2 assay at pH 7.4, 25-30°C, 10 min Gaussia princeps coelenteramide + CO2 + hnu
-
 ?
luciferin + O2 assay at pH 7.4, 25-30°C, 10 min Gaussia princeps ?
-
 ?

Synonyms

Synonyms Comment Organism
Gaussia luciferase
-
 Gaussia princeps
GLase
-
 Gaussia princeps

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 30 assay at Gaussia princeps

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Gaussia princeps