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Literature summary for 1.13.12.5 extracted from
- Renilla luciferase as a reporter to assess SARS-CoV mRNA transcription regulation and efficacy of anti-SARS-CoV agents (2006), Adv. Exp. Med. Biol., 581, 597-600.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | development of a reverse genetic model to characterize the pathway of replication and pathogenesis of the SARS coronavirus. Renilla luciferase is used as a reporter gene and inserted into the backbone of the infectious clone of SARS coronavirus to replace ORF 7a/b (SARS wt-Luc), which is believed to have apoptotic effects on host cells. Recombinant viruses with luciferase constructs are isolated and shown to stably maintain the Renilla luciferase gene and to express subgenomic mRNA encoding luciferase. SARS wt-Luc is a viable virus that allows studies of the effect of subgenomic manipulation on virus efficiacy, both in replication and subgenomic production. This approach offers an alternative to plaque assay analysis in testing the efficiency of anti-SARS agents | Renilla sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Renilla sp. | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Renilla luciferase | - |
Renilla sp. |
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