Literature summary for 1.13.12.4 extracted from

Muh, U.; Massey, V.; Williams, C.H., Jr.
Lactate Monooxygenase. I. Expression of the mycobacterial gene in Escherichia coli and site-directed mutagenesis of lysine 266 (1994), J. Biol. Chem., 269, 7982-7988.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21	Mycolicibacterium smegmatis

Protein Variants

Protein Variants	Comment	Organism
K266M	the rate of reduction with (S)-lactate is decreased compared with that of the wild-type enzyme, the mutant enzyme is virtually inactive	Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-lactate + O2	Mycolicibacterium smegmatis	-	acetate + CO2 + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-lactate + O2	-	Mycolicibacterium smegmatis	acetate + CO2 + H2O	-	?

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)