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Literature summary for 1.13.12.24 extracted from

  • Stepanyuk, G.A.; Golz, S.; Markova, S.V.; Frank, L.A.; Lee, J.; Vysotski, E.S.
    Interchange of aequorin and obelin bioluminescence color is determined by substitution of one active site residue of each photoprotein (2005), FEBS Lett., 579, 1008-1014 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
F88H mutation shifts the bioluminescence maximum from 482 nm for wild-type to 459 nm. 105% of wild-type bioluminescence yield Obelia longissima
F88R mutation shifts the bioluminescence maximum from 482 nm for wild-type to 474 nm. 99% of wild-type bioluminescence yield Obelia longissima
F88W mutation shifts the bioluminescence maximum from 482 nm for wild-type to 477 nm. 100% of wild-type bioluminescence yield Obelia longissima
F88Y mutation shifts the bioluminescence maximum from 482 nm for wild-type to 453 nm. 100% of wild-type bioluminescence yield Obelia longissima
Y82F mutation leads to removal of a H-bond from Tyr82 to the bound coelenteramide, and shifts its bioluminescence from 469 nm for wild-type to 501 nm. Mutant is stable with good activity and expressible in mammalian cells Aequorea victoria

Organism

Organism UniProt Comment Textmining
Aequorea victoria P02592
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Obelia longissima Q27709
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Synonyms

Synonyms Comment Organism
obelin
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Obelia longissima