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Literature summary for 1.13.12.23 extracted from

  • Pojer, F.; Kahlich, R.; Kammerer, B.; Li, S.M.; Heide, L.
    CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis (2003), J. Biol. Chem., 278, 30661-30668 .
    View publication on PubMed
Search for more literature for 1.13.12.23

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not stimulated by addition of 2-oxoglutarate Streptomyces roseochromogenus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces roseochromogenus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a non-heme iron enzyme, Fe2+ is required for enzyme activity. Replacement of Fe2+ with other monovalent or divalent metal ions (1 mM Cu+, Zn2+, Mg2+, or Mn2+) results in almost complete (95-98%) loss of enzymatic activity Streptomyces roseochromogenus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-dimethylallyl-4-hydroxymandelate + O2 Streptomyces roseochromogenus
-
 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
 ?
3-dimethylallyl-4-hydroxymandelate + O2 Streptomyces roseochromogenus DS 12976
-
 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces roseochromogenus Q8GHB1
-
-
Streptomyces roseochromogenus DS 12976 Q8GHB1
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Streptomyces roseochromogenus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dimethylallyl-4-hydroxymandelate + O2
-
 Streptomyces roseochromogenus 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
 ?
3-dimethylallyl-4-hydroxymandelate + O2
-
 Streptomyces roseochromogenus DS 12976 3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 30500, calculated from amino acid sequence Streptomyces roseochromogenus

Synonyms

Synonyms Comment Organism
CloR
-
 Streptomyces roseochromogenus