Literature summary for 1.13.12.2 extracted from

Im, D.; Matsui, D.; Arakawa, T.; Isobe, K.; Asano, Y.; Fushinobu, S.
Ligand complex structures of L-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813 and its conformational change (2018), FEBS open bio, 8, 314-324 .

Search for more literature for 1.13.12.2

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of L-AAO/MOG complexed with L-Lys, L-ornithine, and L-Arg. Residue Asp238 is located at the ceiling of a long hydrophobic pocket and forms a strong interaction with the terminal, positively charged group of the substrates. In the ligand-free structure, there are two channels connecting the active site and solvent, and a short region located at the dimer interface is disordered. In the L-Lys complex structure, a loop region is displaced to plug the channels	Pseudomonas sp. AIU 813

Crystallization (Comment)

Organism

structures of L-AAO/MOG complexed with L-Lys, L-ornithine, and L-Arg. Residue Asp238 is located at the ceiling of a long hydrophobic pocket and forms a strong interaction with the terminal, positively charged group of the substrates. In the ligand-free structure, there are two channels connecting the active site and solvent, and a short region located at the dimer interface is disordered. In the L-Lys complex structure, a loop region is displaced to plug the channels

Pseudomonas sp. AIU 813

Protein Variants

Protein Variants	Comment	Organism
D238A	the interaction of Asp238 with the terminal, positively charged group of the substrates is critical for substrate binding but not for catalytic control between the oxidase/monooxygenase activities	Pseudomonas sp. AIU 813
D238E	mutant displays increased catalytic activities	Pseudomonas sp. AIU 813
D238F	mutant exhibits altered substrate specificity to long hydrophobic substrates	Pseudomonas sp. AIU 813

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.033	-	L-ornithine	wild-type, pH 7, 30°C	Pseudomonas sp. AIU 813
0.043	-	L-lysine	mutant D238E, pH 7, 30°C	Pseudomonas sp. AIU 813
0.061	-	L-lysine	wild-type, pH 7, 30°C	Pseudomonas sp. AIU 813
0.061	-	L-ornithine	mutant D238E, pH 7, 30°C	Pseudomonas sp. AIU 813
0.067	-	L-arginine	wild-type, pH 7, 30°C	Pseudomonas sp. AIU 813
0.072	-	L-lysine	mutant D238F, pH 7, 30°C	Pseudomonas sp. AIU 813
0.09	-	L-arginine	mutant D238F, pH 7, 30°C	Pseudomonas sp. AIU 813
0.26	-	L-lysine	mutant D238A, pH 7, 30°C	Pseudomonas sp. AIU 813
0.31	-	L-ornithine	mutant D238A, pH 7, 30°C	Pseudomonas sp. AIU 813
0.42	-	L-arginine	mutant D238A, pH 7, 30°C	Pseudomonas sp. AIU 813

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. AIU 813	W6JQJ6	bifunctional L-amino acid oxidase/monooxygenase, cf. EC. 1.4.3.2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-arginine + O2	-	Pseudomonas sp. AIU 813	4-guanidinobutanamide + CO2 + H2O	-	?
L-lysine + O2	-	Pseudomonas sp. AIU 813	5-aminopentanamide + CO2 + H2O	-	?
L-ornithine + O2	-	Pseudomonas sp. AIU 813	4-aminobutanamide + CO2 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
L-AAO/MOG	-	Pseudomonas sp. AIU 813

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.29	-	L-ornithine	mutant D238A, pH 7, 30°C	Pseudomonas sp. AIU 813
0.8	-	L-lysine	mutant D238F, pH 7, 30°C	Pseudomonas sp. AIU 813
1.2	-	L-arginine	mutant D238F, pH 7, 30°C	Pseudomonas sp. AIU 813
1.6	-	L-arginine	wild-type, pH 7, 30°C	Pseudomonas sp. AIU 813
2.3	-	L-ornithine	mutant D238E, pH 7, 30°C	Pseudomonas sp. AIU 813
2.7	-	L-lysine	mutant D238A, pH 7, 30°C	Pseudomonas sp. AIU 813
2.8	-	L-arginine	mutant D238A, pH 7, 30°C	Pseudomonas sp. AIU 813
4.5	-	L-lysine	wild-type, pH 7, 30°C	Pseudomonas sp. AIU 813
6.7	-	L-ornithine	wild-type, pH 7, 30°C	Pseudomonas sp. AIU 813
8.1	-	L-lysine	mutant D238E, pH 7, 30°C	Pseudomonas sp. AIU 813

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.94	-	L-ornithine	mutant D238A, pH 7, 30°C	Pseudomonas sp. AIU 813
6.7	-	L-arginine	mutant D238A, pH 7, 30°C	Pseudomonas sp. AIU 813
10	-	L-lysine	mutant D238A, pH 7, 30°C	Pseudomonas sp. AIU 813
11	-	L-lysine	mutant D238F, pH 7, 30°C	Pseudomonas sp. AIU 813
13	-	L-arginine	mutant D238F, pH 7, 30°C	Pseudomonas sp. AIU 813
24	-	L-arginine	wild-type, pH 7, 30°C	Pseudomonas sp. AIU 813
32	-	L-ornithine	mutant D238E, pH 7, 30°C	Pseudomonas sp. AIU 813
74	-	L-lysine	wild-type, pH 7, 30°C	Pseudomonas sp. AIU 813
190	-	L-lysine	mutant D238E, pH 7, 30°C	Pseudomonas sp. AIU 813
200	-	L-ornithine	wild-type, pH 7, 30°C	Pseudomonas sp. AIU 813