Application | Comment | Organism |
---|---|---|
synthesis | installation of an Escherichia coli whole-cell biocatalytic system, environmentally friendly, for industrial production of 5-aminovalerate using recombinant enzymes 5-aminovaleramide amidohydrolase and L-lysine 2-monooxygenase, overview | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
gene davB, recombinant expression of codon-optimized enzyme DavB in Escherichia coli strains BL21(DE3) and BL-22A-RB-YB, coexpression with gene davA, encoding 5-aminovaleramide amidohydrolase | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of an optimized production system for 5-aminovalerate from L-lysine in Escherichia coli by overexpressing genes davA and davB, encoding 5-aminovaleramide amidohydrolase and L-lysine 2-monooxygenase, the effects of induction conditions, reaction temperature, metal ion additives, and cell permeability on the whole-cell biocatalyst system are evaluated to improve biocatalytic efficiency, overview. Presence of Mn2+ and Ca2+ enhances the activity of whole-cell BL-22A-RB-YB system. Increased permeabilization of Escherichia coli BL-22A-RB-YB cells following surfactant treatment with TritonX-100 results in improved L-lysine consumption and 5-aminovalerate synthesis rate | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + O2 | Pseudomonas putida | - |
5-aminopentanamide + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q88QV1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + O2 | - |
Pseudomonas putida | 5-aminopentanamide + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
davB | - |
Pseudomonas putida |
L-lysine 2-monooxygenase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme-bound, dependent on | Pseudomonas putida |