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Literature summary for 1.13.12.2 extracted from

  • Wang, X.; Cai, P.; Chen, K.; Ouyang, P.
    Efficient production of 5-aminovalerate from L-lysine by engineered Escherichia coli whole-cell biocatalysts (2016), J. Mol. Catal. B, 134, 115-121 .
No PubMed abstract available

Application

Application Comment Organism
synthesis installation of an Escherichia coli whole-cell biocatalytic system, environmentally friendly, for industrial production of 5-aminovalerate using recombinant enzymes 5-aminovaleramide amidohydrolase and L-lysine 2-monooxygenase, overview Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
gene davB, recombinant expression of codon-optimized enzyme DavB in Escherichia coli strains BL21(DE3) and BL-22A-RB-YB, coexpression with gene davA, encoding 5-aminovaleramide amidohydrolase Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information generation of an optimized production system for 5-aminovalerate from L-lysine in Escherichia coli by overexpressing genes davA and davB, encoding 5-aminovaleramide amidohydrolase and L-lysine 2-monooxygenase, the effects of induction conditions, reaction temperature, metal ion additives, and cell permeability on the whole-cell biocatalyst system are evaluated to improve biocatalytic efficiency, overview. Presence of Mn2+ and Ca2+ enhances the activity of whole-cell BL-22A-RB-YB system. Increased permeabilization of Escherichia coli BL-22A-RB-YB cells following surfactant treatment with TritonX-100 results in improved L-lysine consumption and 5-aminovalerate synthesis rate Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 Pseudomonas putida
-
5-aminopentanamide + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88QV1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + O2
-
Pseudomonas putida 5-aminopentanamide + CO2 + H2O
-
?

Synonyms

Synonyms Comment Organism
davB
-
Pseudomonas putida
L-lysine 2-monooxygenase
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD enzyme-bound, dependent on Pseudomonas putida