Any feedback?
Literature summary for 1.13.12.19 extracted from
- Expression of NADH-oxidases enhances ethylene productivity in Saccharomyces cerevisiae expressing the bacterial EFE (2017), Biotechnol. Bioprocess Eng., 22, 195-199 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons with Pseudomonas digitatum and Pseudomonas chrysogenum, recombinant expression in Saccharomyces cerevisiae | Pseudomonas savastanoi pv. phaseolicola |
| DNA and amino acid sequence determination and analysis, sequence comparisons with Pseudomonas digitatum and Pseudomonas chrysogenum, recombinant expression in Saccharomyces cerevisiae | Pseudomonas syringae pv. pisi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A199G | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| A199G | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas syringae pv. pisi |
| C280F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| C280F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas syringae pv. pisi |
| D191A | site-directed mutagenesis, inactive mutant | Pseudomonas savastanoi pv. phaseolicola |
| D191A | site-directed mutagenesis, inactive mutant | Pseudomonas syringae pv. pisi |
| E235D | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| E235D | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Pseudomonas syringae pv. pisi |
| F278Y | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| F278Y | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Pseudomonas syringae pv. pisi |
| H189A | site-directed mutagenesis, inactive mutant | Pseudomonas savastanoi pv. phaseolicola |
| H189A | site-directed mutagenesis, inactive mutant | Pseudomonas syringae pv. pisi |
| H233A | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| H233A | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Pseudomonas syringae pv. pisi |
| H268A | site-directed mutagenesis, inactive mutant | Pseudomonas savastanoi pv. phaseolicola |
| H268A | site-directed mutagenesis, inactive mutant | Pseudomonas syringae pv. pisi |
| I254M | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| I254M | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Pseudomonas syringae pv. pisi |
| I304N | site-directed mutagenesis, inactive mutant | Pseudomonas savastanoi pv. phaseolicola |
| I304N | site-directed mutagenesis, inactive mutant | Pseudomonas syringae pv. pisi |
| I322V | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| I322V | site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme | Pseudomonas syringae pv. pisi |
| L22M | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| L22M | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Pseudomonas syringae pv. pisi |
| additional information | a loop deletion mutant is inactive | Pseudomonas savastanoi pv. phaseolicola |
| additional information | a loop deletion mutant is inactive | Pseudomonas syringae pv. pisi |
| R236S | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| R236S | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas syringae pv. pisi |
| V172T | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| V172T | site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme | Pseudomonas syringae pv. pisi |
| V212Y/E213S | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas savastanoi pv. phaseolicola |
| V212Y/E213S | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Pseudomonas syringae pv. pisi |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | dependent on, residues H189, D191 and H268 are responsible for binding the Fe(II) ligand | Pseudomonas savastanoi pv. phaseolicola |  |
| Fe2+ | dependent on, residues H189, D191 and H268 are responsible for binding the Fe(II) ligand | Pseudomonas syringae pv. pisi | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + O2 | Pseudomonas savastanoi pv. phaseolicola | - |
ethylene + 3 CO2 + H2O | - |
? | |
| 2-oxoglutarate + O2 | Pseudomonas syringae pv. pisi | - |
ethylene + 3 CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas savastanoi pv. phaseolicola | P32021 | - |
- |
| Pseudomonas syringae pv. pisi | Q9Z3T0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + O2 | - |
Pseudomonas savastanoi pv. phaseolicola | ethylene + 3 CO2 + H2O | - |
? | |
| 2-oxoglutarate + O2 | - |
Pseudomonas syringae pv. pisi | ethylene + 3 CO2 + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EFE | - |
Pseudomonas savastanoi pv. phaseolicola |
| EFE | - |
Pseudomonas syringae pv. pisi |
| ethylene forming enzyme | - |
Pseudomonas savastanoi pv. phaseolicola |
| ethylene forming enzyme | - |
Pseudomonas syringae pv. pisi |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to a subclass of 2-oxoglutarate/Fe(II) dependent dioxygenases, structure-function analysis of the ethylene forming subclass of 2-oxoglutarate/Fe(II)-dependent dioxygenases, overview | Pseudomonas syringae pv. pisi |
| evolution | the enzyme belongs to a subclass of 2-oxoglutarate/Fe(II) dependent dioxygenases, structure-function analysis of the ethylene forming subclass of 2-oxoglutarate/Fe(II)-dependent dioxygenases,overview | Pseudomonas savastanoi pv. phaseolicola |
| additional information | three of the amino acids correlating with ethylene production are located in the predicted 2-oxoglutarate binding domain, a protein domain specific for the EFE-class that is essential for activity. Residues H189, D191 and H268 are responsible for binding the Fe(II) ligand | Pseudomonas savastanoi pv. phaseolicola |
| additional information | three of the amino acids correlating with ethylene production are located in the predicted 2-oxoglutarate binding domain, a protein domain specific for the EFE-class that is essential for activity. Residues H189, D191 and H268 are responsible for binding the Fe(II) ligand | Pseudomonas syringae pv. pisi |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
