Literature summary for 1.13.12.19 extracted from

Veetil, V.P.; Angermayr, S.A.; Hellingwerf, K.J.
Ethylene production with engineered Synechocystis sp PCC 6803 strains (2017), Microb. Cell Fact., 16, 34 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene efe, recombinant enzyme expression in Synechocystis sp. strain PCC 6803, stable ethylene production through the integration of a codon-optimized version of the efe gene under control of the Ptrc promoter and the core Shine-Dalgarno sequence (5'-AGGAGG-3) as the ribosome-binding site, at the slr0168 neutral site. Increase in ethylene production is achieved twofold by RBS screening, improvement of ethylene production from a single gene copy of efe, using multiple tandem promoters and by putting our best construct on an RSF1010-based broad-hostself-replicating plasmid, which has a higher copy number than the genome	Pseudomonas savastanoi pv. phaseolicola

Cloned (Comment)

Organism

gene efe, recombinant enzyme expression in Synechocystis sp. strain PCC 6803, stable ethylene production through the integration of a codon-optimized version of the efe gene under control of the Ptrc promoter and the core Shine-Dalgarno sequence (5'-AGGAGG-3) as the ribosome-binding site, at the slr0168 neutral site. Increase in ethylene production is achieved twofold by RBS screening, improvement of ethylene production from a single gene copy of efe, using multiple tandem promoters and by putting our best construct on an RSF1010-based broad-hostself-replicating plasmid, which has a higher copy number than the genome

Pseudomonas savastanoi pv. phaseolicola

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a glycogen-synthesis knockout mutant (DELTAglgC) to raise the intracellular amounts of the key Efe substrate, 2-oxoglutarate, from which ethylene is formed. Introduction of the ethylene biosynthetic pathway in mutant DELTAglgC. Under nitrogen limiting conditions, the glycogen knockout strain has increased intracellular 2-oxoglutarate levels, but the ethylene production is lower in the mutant strain than in the wild-type strain	Pseudomonas savastanoi pv. phaseolicola

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-oxoglutarate + O2	Pseudomonas savastanoi pv. phaseolicola	-	ethylene + 3 CO2 + H2O	-	?
2-oxoglutarate + O2	Pseudomonas savastanoi pv. phaseolicola PK2	-	ethylene + 3 CO2 + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas savastanoi pv. phaseolicola	Q549K5	the Kudzu strain	-
Pseudomonas savastanoi pv. phaseolicola PK2	Q549K5	the Kudzu strain	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + O2	-	Pseudomonas savastanoi pv. phaseolicola	ethylene + 3 CO2 + H2O	-	?
2-oxoglutarate + O2	-	Pseudomonas savastanoi pv. phaseolicola PK2	ethylene + 3 CO2 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
ethylene-forming enzyme	-	Pseudomonas savastanoi pv. phaseolicola

General Information

General Information	Comment	Organism
physiological function	the ethylene-forming enzyme (Efe) from Pseudomonas syringae pv. phaseolicola PK2 (the Kudzu strain) catalyzes the conversion of the ubiquitous tricarboxylic acid cycle intermediate 2-oxoglutarate into ethylene	Pseudomonas savastanoi pv. phaseolicola