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Literature summary for 1.13.12.18 extracted from

  • Suzuki-Ogoh, C.; Wu, C.; Ohmiya, Y.
    C-terminal region of the active domain enhances enzymatic activity in dinoflagellate luciferase (2008), Photochem. Photobiol. Sci., 7, 208-211.
    View publication on PubMed
Search for more literature for 1.13.12.18

Cloned(Commentary)

Cloned (Comment) Organism
deletion mutants for N- or C-terminal regions of domain 3 of the luciferase are expressed in Escherichia coli BL21(DE3) cells Lingulodinium polyedra

Protein Variants

Protein Variants Comment Organism
additional information deletion mutants for N-terminal region of domain 3 of the luciferase show above 20% of wild type activity, the activities of C-terminal deleted mutants decrease drastically to below 1% of wild type Lingulodinium polyedra

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, SDS-PAGE Lingulodinium polyedra

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dinoflagellate luciferin + O2 Lingulodinium polyedra
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lingulodinium polyedra
-
 formerly known as Gonyaulax polyedra
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Lingulodinium polyedra

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dinoflagellate luciferin + O2
-
 Lingulodinium polyedra ?
-
 ?
dinoflagellate luciferin + O2
-
 Lingulodinium polyedra oxidized dinoflagellate luciferin + H2O + hv
-
 ?

Subunits

Subunits Comment Organism
homotrimer 2 * 42000, SDS-PAGE Lingulodinium polyedra

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 the optimum pH of Lingulodinium polyedrum luciferase and for each of its domains is approximately 6.0 Lingulodinium polyedra
6.5
-
 Lingulodinium polyedrum luciferase domain 3 Lingulodinium polyedra