BRENDA - Enzyme Database show
show all sequences of 1.13.12.17

Nonenzymatic oxidative steps accompanying action of the cytochrome P450 enzymes StaP and RebP in the biosynthesis of staurosporine and rebeccamycin

Howard-Jones, A.R.; Walsh, C.T.; J. Am. Chem. Soc. 129, 11016-11007 (2007)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+
Streptomyces longisporoflavus
-
dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces longisporoflavus
Q06IS2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+
-
698455
Streptomyces longisporoflavus
dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+
-
-
-
?
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+
StaP/RebP is simply responsible for the two-electron intramolecular aryl-aryl coupling of dichlorochromopyrrolate to give the six-ring intermediate 5,7-dicarboxy-12,13-dihydro-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole with all subsequent steps occurring nonenzymatically in solution. The study on the nonenzymatic steps following StaP-mediated aryl-aryl bond formation corroborates the proposed role of RebC (and by extension StaC) in intercepting and redirecting intermediates en route to the aglycones, K252c, 7-hydroxy-K252c, and arcyriaflavin A
698455
Streptomyces longisporoflavus
dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+
Streptomyces longisporoflavus
-
dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+
-
698455
Streptomyces longisporoflavus
dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+
-
-
-
?
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+
StaP/RebP is simply responsible for the two-electron intramolecular aryl-aryl coupling of dichlorochromopyrrolate to give the six-ring intermediate 5,7-dicarboxy-12,13-dihydro-5H-indolo[2,3-a]pyrrolo[3,4-c]carbazole with all subsequent steps occurring nonenzymatically in solution. The study on the nonenzymatic steps following StaP-mediated aryl-aryl bond formation corroborates the proposed role of RebC (and by extension StaC) in intercepting and redirecting intermediates en route to the aglycones, K252c, 7-hydroxy-K252c, and arcyriaflavin A
698455
Streptomyces longisporoflavus
dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+
-
-
-
?
Other publictions for EC 1.13.12.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
698497
Wang
Theoretical and experimental s ...
Streptomyces longisporoflavus
J. Am. Chem. Soc.
131
6748-6762
2009
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698474
Wang
Electron transfer activation o ...
Streptomyces longisporoflavus
J. Am. Chem. Soc.
130
7170-7171
2008
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698455
Howard-Jones
Nonenzymatic oxidative steps a ...
Streptomyces longisporoflavus
J. Am. Chem. Soc.
129
11016-11007
2007
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700932
Makino
Crystal structures and catalyt ...
Streptomyces sp., Streptomyces sp. TP-A0274
Proc. Natl. Acad. Sci. USA
104
11591-11596
2007
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698452
Howard-Jones
Staurosporine and rebeccamycin ...
Streptomyces longisporoflavus
J. Am. Chem. Soc.
128
12289-12298
2006
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700928
Sanchez
Rohr, J.; Mendez, C.; Salas, J ...
Streptomyces albus
Proc. Natl. Acad. Sci. USA
102
461-466
2004
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