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Literature summary for 1.13.12.16 extracted from
- Peculiarities of nitronate monooxygenases and perspectives for in vivo and in vitro applications (2021), Appl. Microbiol. Biotechnol., 105, 8019-8032.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.34 | - |
propionate-3-nitronate | pH and temperature not specified in the publication | Neurospora crassa |  |
| 0.58 | - |
3-nitropropionate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.4 | - |
hexyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 3.1 | - |
ethyl nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 5 | - |
propyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 10 | - |
butyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 24 | - |
1-Nitropropane | pH and temperature not specified in the publication | Neurospora crassa | |
| 29 | - |
nitroethane | pH and temperature not specified in the publication | Neurospora crassa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | Q01284 | - |
- |
| Neurospora crassa DSM 1257 | Q01284 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-nitropropane + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1-nitropropane + O2 | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| 3-nitropropionate + O2 | - |
Neurospora crassa | ? | - |
? | |
| 3-nitropropionate + O2 | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| butyl 1-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| ethyl nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| hexyl 1-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| additional information | the enzyme can catalyze the oxidation of neutral or anionic nitroalkanes and alkyl nitronates to their corresponding carbonyl and nitro compounds | Neurospora crassa | ? | - |
? | |
| additional information | the enzyme can catalyze the oxidation of neutral or anionic nitroalkanes and alkyl nitronates to their corresponding carbonyl and nitro compounds | Neurospora crassa DSM 1257 | ? | - |
? | |
| nitroethane + O2 | - |
Neurospora crassa | ? | - |
? | |
| nitroethane + O2 | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| pentyl 1-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| propionate-3-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| propionate-3-nitronate + O2 | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| propyl 1-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Neurospora crassa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ncd2 | - |
Neurospora crassa |
| NMO | - |
Neurospora crassa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.4 | - |
3-nitropropionate | pH and temperature not specified in the publication | Neurospora crassa | |
| 14 | - |
nitroethane | pH and temperature not specified in the publication | Neurospora crassa | |
| 20 | - |
1-Nitropropane | pH and temperature not specified in the publication | Neurospora crassa | |
| 22 | - |
hexyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 51 | - |
ethyl nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 55 | - |
butyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 60 | - |
propyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 430 | - |
propionate-3-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Neurospora crassa | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
1-Nitropropane | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.2 | - |
pentyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 5.6 | - |
butyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 7.6 | - |
3-nitropropionate | pH and temperature not specified in the publication | Neurospora crassa | |
| 11.4 | - |
propyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 16 | - |
hexyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 16.5 | - |
ethyl nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1300 | - |
propionate-3-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
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