Literature summary for 1.13.12.16 extracted from

Ha, J.Y.; Min, J.Y.; Lee, S.K.; Kim, H.S.; Kim, d.o.J.; Kim, K.H.; Lee, H.H.; Kim, H.K.; Yoon, H.J.; Suh, S.W.
Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base (2006), J. Biol. Chem., 281, 18660-18667.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli C41(DE3) cells	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method using 10% (w/v) polyethylene glycol monomethyl ether 5000	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
H152A	His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon	Pseudomonas aeruginosa
H152A	site-directed mutagenesis. His152 likely functions as the catalytic base that initiates oxidation of neutral substrates by abstracting a proton from the alpha-carbon	Pseudomonas aeruginosa
S288A	mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli	Pseudomonas aeruginosa
S288A	site-directed mutagenesis. Mutant enzyme forms inclusion bodies when overexpressed in Escherichia coli	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibition by valeric acid and EDTA	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	dynamic light scattering analysis	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9I4V0	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiLoad XK 16 Superdex 200 prep-grade column gel filtration and Mono Q HR5/5 column chromatography	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-nitropropane + O2	-	Pseudomonas aeruginosa	acetone + HNO2	-	?
2-nitropropane + O2	anionic form	Pseudomonas aeruginosa	acetone + HNO2	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 37000, dynamic light scattering analysis	Pseudomonas aeruginosa
monomer	monomer 1 * 37000, dynamic light scattering analysis	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
2-nitropropane dioxygenase	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)