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Literature summary for 1.13.12.16 extracted from

  • Francis, K.; Gadda, G.
    Probing the chemical steps of nitroalkane oxidation catalyzed by 2-nitropropane dioxygenase with solvent viscosity, pH, and substrate kinetic isotope effects (2006), Biochemistry, 45, 13889-13898.
    View publication on PubMed
Search for more literature for 1.13.12.16

Inhibitors

Inhibitors Comment Organism Structure
m-Nitrobenzoate
-
 Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 O2 with ethyl nitronate at pH 8.0 and 30°C Neurospora crassa
0.005
-
 O2 with nitroethane at pH 9.0 and 30°C Neurospora crassa
0.005
-
 O2 with ethyl nitronate at pH 8.0 and 30°C; with nitroethane at pH 9.0 and 30°C Neurospora crassa
0.01
-
 O2 with nitroethane at pH 6.0 and 30°C Neurospora crassa
0.02
-
 O2 with either ethyl nitronate or nitroethane at pH 6.0 and 30°C Neurospora crassa
0.045
-
 O2 with ethyl nitronate at pH 9.0 and 30°C Neurospora crassa
3.4
-
 ethyl nitronate at pH 8.0 and 30°C Neurospora crassa
5.1
-
 ethyl nitronate at pH 6.0 and 30°C Neurospora crassa
9.5
-
 ethyl nitronate at pH 9.0 and 30°C Neurospora crassa
13
-
 nitroethane at pH 9.0 and 30°C Neurospora crassa
19
-
 nitroethane at pH 8.0 and 30°C Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl nitronate + O2
-
 Neurospora crassa ? + nitrite
-
 ?
ethyl nitronate + O2 anionic form of the substrate Neurospora crassa ? + nitrite
-
 ?
nitroethane + O2 neutral form of the substrate Neurospora crassa acetaldehyde + HNO2
-
 ?
nitroethane + O2 + H2O
-
 Neurospora crassa ethanal + nitrite + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
2-nitropropane dioxygenase
-
 Neurospora crassa
NAO
-
 Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.9
-
 nitroethane at pH 9.0 and 30°C Neurospora crassa
11
-
 nitroethane at pH 8.0 and 30°C Neurospora crassa
25
-
 ethyl nitronate at pH 9.0 and 30°C Neurospora crassa
57
-
 ethyl nitronate at pH 8.0 and 30°C Neurospora crassa
130
-
 ethyl nitronate at pH 6.0 and 30°C Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
6 9 investigation of the pH effects on the kcat/Km-values Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
FMN dependent Neurospora crassa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information investigation of the pH effects on the kcat/Km-values Neurospora crassa
0.04
-
 nitroethane at pH 6.0 and 30°C Neurospora crassa
0.375
-
 nitroethane at pH 9.0 and 30°C Neurospora crassa
0.56
-
 nitroethane at pH 8.0 and 30°C Neurospora crassa
2.5
-
 ethyl nitronate at pH 9.0 and 30°C Neurospora crassa
16.9
-
 ethyl nitronate at pH 8.0 and 30°C Neurospora crassa
25.4
-
 ethyl nitronate at pH 6.0 and 30°C Neurospora crassa