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Literature summary for 1.13.12.16 extracted from

  • Gorlatova, N.; Tchorzewski, M.; Kurihara, T.; Soda, K.; Esaki, N.
    Purification, characterization, and mechanism of a flavin mononucleotide-dependent 2-nitropropane dioxygenase from Neurospora crassa (1998), Appl. Environ. Microbiol., 64, 1029-1033.
    View publication on PubMed
Search for more literature for 1.13.12.16

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, relative activity remaining 2% Neurospora crassa
cytochrome c 0.04 mM, relative activity remaining 34% Neurospora crassa
dithiothreitol 1 mM, complete inhibition Neurospora crassa
EDTA 1 mM, relative activity remaining 95% Neurospora crassa
GSH 5 mM, relative activity remaining 28% Neurospora crassa
iodoacetate 1 mM, complete inhibition Neurospora crassa
Nitro blue tetrazolium 2.5 mM, relative activity remaining 10% Neurospora crassa
Superoxide dismutase complete inhibition Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 nitroethane neutral form of substrate Neurospora crassa
3.1
-
 2-Nitropropane anionic form of substrate Neurospora crassa
3.1
-
 propyl-2-nitronate anionic form of substrate Neurospora crassa
6
-
 2-Nitropropane anionic form of substrate Neurospora crassa
6
-
 ethyl nitronate anionic form of substrate Neurospora crassa
8.3
-
 1-Nitropropane anionic form of substrate Neurospora crassa
8.3
-
 propyl-1-nitronate anionic form of substrate Neurospora crassa
20
-
 1-Nitropropane neutral form of substrate Neurospora crassa
23
-
 nitroethane neutral form of substrate Neurospora crassa
33
-
 2-Nitropropane neutral form of substrate Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Neurospora crassa
70000
-
 gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-
Neurospora crassa
-
 ATCC 10337
-

Purification (Commentary)

Purification (Comment) Organism
-
 Neurospora crassa
to homogeneity Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10000
-
-
 Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxybutyl-2-nitronate + O2
-
 Neurospora crassa 3-hydroxy-butane-2-one + HNO2
-
 ?
1-hydroxyethyl-2-nitronate + O2
-
 Neurospora crassa glycoaldehyde + HNO2
-
 ?
1-nitropentane + O2 3% of the activity with 2-nitropropane Neurospora crassa ? + HNO2
-
 ?
1-nitropropane + O2 21% of the activity with 2-nitropropane Neurospora crassa propionaldehyde + HNO2
-
 ?
2-hydroxypentyl-3-nitronate + O2
-
 Neurospora crassa 2-hydroxy-pentane-3-one + HNO2
-
 ?
2-nitroethanol + O2 13% of the activity with 2-nitropropane Neurospora crassa glycoaldehyde + HNO2
-
 ?
2-nitropropane + O2
-
 Neurospora crassa acetone + HNO2
-
 ?
3-nitro-2-butanol + O2 14% of the activity with 2-nitropropane Neurospora crassa 3-hydroxy-butane-2-one + HNO2
-
 ?
3-nitro-2-pentanol + O2 20% of the activity with 2-nitropropane Neurospora crassa 2-hydroxy-pentane-3-one + HNO2
-
 ?
3-nitropropionic acid + O2 12% of the activity with 2-nitropropane Neurospora crassa ?
-
 ?
cyclohexyl nitronate + O2
-
 Neurospora crassa ? + HNO2
-
 ?
ethyl nitronate + O2
-
 Neurospora crassa acetaldehyde + HNO2
-
 ?
additional information anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds Neurospora crassa ?
-
 ?
additional information anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds. Measuring nitrite production with 20 mM anionic nitro compounds as substrates Neurospora crassa ?
-
 ?
nitrocyclohexane + O2 2% of the activity with 2-nitropropane Neurospora crassa ? + HNO2
-
 ?
nitroethane + O2 27% of the activity with 2-nitropropane Neurospora crassa acetaldehyde + HNO2
-
 ?
pentyl-1-nitronate + O2
-
 Neurospora crassa ? + HNO2
-
 ?
propionate-3-nitronate + O2
-
 Neurospora crassa ?
-
 ?
propyl-1-nitronate + O2
-
 Neurospora crassa propionaldehyde + HNO2
-
 ?
propyl-2-nitronate + O2
-
 Neurospora crassa acetone + HNO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Neurospora crassa

Synonyms

Synonyms Comment Organism
2-nitropropane dioxygenase
-
 Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32
-
 nitroethane neutral form of substrate Neurospora crassa
35
-
 1-Nitropropane neutral form of substrate Neurospora crassa
160
-
 2-Nitropropane neutral form of substrate Neurospora crassa
1300
-
 1-Nitropropane anionic form of substrate Neurospora crassa
1300
-
 propyl-1-nitronate anionic form of substrate Neurospora crassa
2000
-
 nitroethane anionic form of substrate Neurospora crassa
2000
-
 ethyl nitronate anionic form of substrate Neurospora crassa
6400
-
 2-Nitropropane anionic form of substrate Neurospora crassa
6400
-
 propyl-2-nitronate anionic form of substrate Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
FMN 2 mol of FMN per mol of subunit Neurospora crassa
FMN dependent, enzyme contains flavin mononucleotide as a prosthetic group, 2 mol of FMN per mol of subunit Neurospora crassa