BRENDA - Enzyme Database show
show all sequences of 1.13.12.15

Purification and characterization of 3,4-dihydroxyphenylalanine oxidative deaminase from Rhodobacter sphaeroides OU5

Ranjith, N.K.; Ramana, C.h.V.; Sasikala, C.H.; Can. J. Microbiol. 54, 829-834 (2008)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Rhodobacter sphaeroides
NADH
-
Rhodobacter sphaeroides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Km value for 3,4-dihydroxy-L-phenylalanine is 11.84 microM
Rhodobacter sphaeroides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
190000
-
gel filtration
Rhodobacter sphaeroides
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter sphaeroides
-
-
-
Rhodobacter sphaeroides OU5
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rhodobacter sphaeroides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4-dihydroxy-L-phenylalanine + O2
-
685904
Rhodobacter sphaeroides
3,4-dihydroxyphenylpyruvate + NH3
-
-
-
?
3,4-dihydroxy-L-phenylalanine + O2
-
685904
Rhodobacter sphaeroides OU5
3,4-dihydroxyphenylpyruvate + NH3
-
-
-
?
glycine + O2
-
685904
Rhodobacter sphaeroides
oxoacetic acid + NH3
30% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
glycine + O2
-
685904
Rhodobacter sphaeroides OU5
oxoacetic acid + NH3
30% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-alanine + O2
-
685904
Rhodobacter sphaeroides
2-oxo-propanoic acid + NH3
20% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-alanine + O2
-
685904
Rhodobacter sphaeroides OU5
2-oxo-propanoic acid + NH3
20% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-glutamate + O2
-
685904
Rhodobacter sphaeroides
2-oxopentanedioic acid + NH3
60% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-glutamate + O2
-
685904
Rhodobacter sphaeroides OU5
2-oxopentanedioic acid + NH3
60% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-phenylalanine + O2
-
685904
Rhodobacter sphaeroides
2-oxo-3-phenylpropanoic acid + NH3
60% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-phenylalanine + O2
-
685904
Rhodobacter sphaeroides OU5
2-oxo-3-phenylpropanoic acid + NH3
60% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-tryptophan + O2
-
685904
Rhodobacter sphaeroides
3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3
50% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-tyrosine + O2
-
685904
Rhodobacter sphaeroides
3-(4-hydroxyphenyl)-2-oxopropanoic acid + NH3
80% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
Subunits
Subunits
Commentary
Organism
pentamer
five different subunits of 54000 Dalton, 43000 Dalton, 34000 Dalton, 25000 Dalton, and 22000 Dalton, SDS-PAGE
Rhodobacter sphaeroides
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.68
-
3,4-dihydroxy-L-phenylalanine
pH 7.8, 30C
Rhodobacter sphaeroides
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor required
Rhodobacter sphaeroides
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
additional information
-
IC50 value for NADH is 35 microM per l, IC50 value for 2-oxoglutarate is 25 microM per l
Rhodobacter sphaeroides
additional information
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor required
Rhodobacter sphaeroides
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
additional information
-
IC50 value for NADH is 35 microM per l, IC50 value for 2-oxoglutarate is 25 microM per l
Rhodobacter sphaeroides
additional information
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
-
Rhodobacter sphaeroides
NADH
-
Rhodobacter sphaeroides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Km value for 3,4-dihydroxy-L-phenylalanine is 11.84 microM
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
190000
-
gel filtration
Rhodobacter sphaeroides
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4-dihydroxy-L-phenylalanine + O2
-
685904
Rhodobacter sphaeroides
3,4-dihydroxyphenylpyruvate + NH3
-
-
-
?
3,4-dihydroxy-L-phenylalanine + O2
-
685904
Rhodobacter sphaeroides OU5
3,4-dihydroxyphenylpyruvate + NH3
-
-
-
?
glycine + O2
-
685904
Rhodobacter sphaeroides
oxoacetic acid + NH3
30% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
glycine + O2
-
685904
Rhodobacter sphaeroides OU5
oxoacetic acid + NH3
30% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-alanine + O2
-
685904
Rhodobacter sphaeroides
2-oxo-propanoic acid + NH3
20% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-alanine + O2
-
685904
Rhodobacter sphaeroides OU5
2-oxo-propanoic acid + NH3
20% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-glutamate + O2
-
685904
Rhodobacter sphaeroides
2-oxopentanedioic acid + NH3
60% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-glutamate + O2
-
685904
Rhodobacter sphaeroides OU5
2-oxopentanedioic acid + NH3
60% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-phenylalanine + O2
-
685904
Rhodobacter sphaeroides
2-oxo-3-phenylpropanoic acid + NH3
60% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-phenylalanine + O2
-
685904
Rhodobacter sphaeroides OU5
2-oxo-3-phenylpropanoic acid + NH3
60% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-tryptophan + O2
-
685904
Rhodobacter sphaeroides
3-(1H-indol-3-yl)-2-oxopropanoic acid + NH3
50% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
L-tyrosine + O2
-
685904
Rhodobacter sphaeroides
3-(4-hydroxyphenyl)-2-oxopropanoic acid + NH3
80% of the activity with 3,4-dihydroxy-L-phenylalanine
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
pentamer
five different subunits of 54000 Dalton, 43000 Dalton, 34000 Dalton, 25000 Dalton, and 22000 Dalton, SDS-PAGE
Rhodobacter sphaeroides
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.68
-
3,4-dihydroxy-L-phenylalanine
pH 7.8, 30C
Rhodobacter sphaeroides
Other publictions for EC 1.13.12.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
685904
Ranjith
Purification and characterizat ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides OU5
Can. J. Microbiol.
54
829-834
2008
-
-
-
-
-
-
2
1
-
-
1
-
-
4
-
-
1
-
-
-
-
-
12
1
-
-
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
-
1
2
-
1
-
-
1
-
-
-
-
1
-
-
-
-
12
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
690973
Bertoldi
Insights into the mechanism of ...
Sus scrofa
Biochemistry
47
7187-7195
2008
-
-
1
-
2
-
1
-
-
-
-
4
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
4
-
-
-
1
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-