BRENDA - Enzyme Database show
show all sequences of 1.13.11.B6

Secretion of two novel enzymes, manganese 9S-lipoxygenase and epoxy alcohol synthase, by the rice pathogen Magnaporthe salvinii

Wennman, A.; Oliw, E.H.; J. Lipid Res. 54, 762-775 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant expression in Pichia pastoris
Nakataea oryzae
Engineering
Amino acid exchange
Commentary
Organism
L350M
site-directed mutagenesis, the mutant shows increased specificity for 13S-lipoxygenation, EC 1.13.11.12, altered substrate specificity/regiospecific activity, overview
Nakataea oryzae
additional information
replacement Leu350 in 9S-MnLOX and changes the regiospecific oxidation of 18:2 n-6 in a consistent way, but the n-3 double bond of 18:3 n-3 can reduce this effect
Nakataea oryzae
General Stability
General Stability
Organism
9S-LOX activity is less robust during expression and purification, possibly due to proteolysis, stability is not improved by addition of 0.001 mM pepstatin A, 0.001 mM leupeptin, or 1 mM EDTA to the growth medium
Nakataea oryzae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0037
-
(9Z,12Z)-octadeca-9,12-dienoic acid
pH 9.0, 22C
Nakataea oryzae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
the enzyme is secreted
Nakataea oryzae
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
Mn:Fe ratio is 1:0.05
Nakataea oryzae
Mn2+
manganese 9S-lipoxygenase, 9S-LOX contains catalytic manganese, Mn:protein ratio is about 0.2:1, while the Mn:Fe ratio is 1:0.05
Nakataea oryzae
additional information
the catalytic metal of the 9S-LOX is manganese and not iron
Nakataea oryzae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66000
-
x * 80000-90000, recombinant glycosylated enzyme, SDS-PAGE, x * 66000, recombinant deglycosylated enzyme, SDS-PAGE
Nakataea oryzae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nakataea oryzae
-
-
-
Nakataea oryzae CBS 288.54
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Pichia pastoris by hydrophobic interaction chromatography, dialfiltration, and gel filtration
Nakataea oryzae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Nakataea oryzae
-
Storage Stability
Storage Stability
Organism
purified recombinant 9S-MnLOX can be stored for several weeks at 4C with retained activity
Nakataea oryzae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(9E,11Z,15E)-octadeca-9,11,15-trienoic acid + O2
-
725658
Nakataea oryzae
?
-
-
-
?
(9E,11Z,15E)-octadeca-9,11,15-trienoic acid + O2
-
725658
Nakataea oryzae CBS 288.54
?
-
-
-
?
(9Z,12Z)-octadeca-9,12-dienoic acid + O2
alpha-linoleate is converted via two intermediates, (9Z,11S,12Z)-11-hydroperoxy-9,12-octadecenoate and (9Z,11E,13R)-13-hydroperoxy-9,12-octadecadienoate, which are isomerized to the end product, probably after oxidation to peroxyl radicals, beta-fragmentation, and oxygen insertion at C-9
725658
Nakataea oryzae
(9S,10E,12Z)-9-hydroperoxy-octadeca-10,12-dienoate
-
-
-
?
(9Z,12Z)-octadeca-9,12-dienoic acid + O2
alpha-linoleate is converted via two intermediates, (9Z,11S,12Z)-11-hydroperoxy-9,12-octadecenoate and (9Z,11E,13R)-13-hydroperoxy-9,12-octadecadienoate, which are isomerized to the end product, probably after oxidation to peroxyl radicals, beta-fragmentation, and oxygen insertion at C-9
725658
Nakataea oryzae CBS 288.54
(9S,10E,12Z)-9-hydroperoxy-octadeca-10,12-dienoate
-
-
-
?
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2
gamma-linoleate is oxidized at C-9, C-11, and C-13
725658
Nakataea oryzae
?
-
-
-
?
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2
gamma-linoleate is oxidized at C-9, C-11, and C-13
725658
Nakataea oryzae CBS 288.54
?
-
-
-
?
additional information
cf. EC 1.13.11.45, LC- and GC-MS analysis product analysis, overview. Authentic (11S)-hydroperoxyoctadecadienoate is rapidly transformed to 9-hydroperoxyoctadecadienoate by 9S-MnLOX, and only traces of 13-hydroperoxyoctadecadienoate. 9S-MnLOX also partly transforms authentic (13R)-hydroperoxyoctadecadienoate to (9S)-hydroperoxyoctadecadienoate
725658
Nakataea oryzae
?
-
-
-
-
additional information
cf. EC 1.13.11.45, LC- and GC-MS analysis product analysis, overview. Authentic (11S)-hydroperoxyoctadecadienoate is rapidly transformed to 9-hydroperoxyoctadecadienoate by 9S-MnLOX, and only traces of 13-hydroperoxyoctadecadienoate. 9S-MnLOX also partly transforms authentic (13R)-hydroperoxyoctadecadienoate to (9S)-hydroperoxyoctadecadienoate
725658
Nakataea oryzae CBS 288.54
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 80000-90000, recombinant glycosylated enzyme, SDS-PAGE, x * 66000, recombinant deglycosylated enzyme, SDS-PAGE
Nakataea oryzae
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at
Nakataea oryzae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.3
-
(9Z,12Z)-octadeca-9,12-dienoic acid
pH 9.0, 22C
Nakataea oryzae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Nakataea oryzae
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression in Pichia pastoris
Nakataea oryzae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
L350M
site-directed mutagenesis, the mutant shows increased specificity for 13S-lipoxygenation, EC 1.13.11.12, altered substrate specificity/regiospecific activity, overview
Nakataea oryzae
additional information
replacement Leu350 in 9S-MnLOX and changes the regiospecific oxidation of 18:2 n-6 in a consistent way, but the n-3 double bond of 18:3 n-3 can reduce this effect
Nakataea oryzae
General Stability (protein specific)
General Stability
Organism
9S-LOX activity is less robust during expression and purification, possibly due to proteolysis, stability is not improved by addition of 0.001 mM pepstatin A, 0.001 mM leupeptin, or 1 mM EDTA to the growth medium
Nakataea oryzae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0037
-
(9Z,12Z)-octadeca-9,12-dienoic acid
pH 9.0, 22C
Nakataea oryzae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
the enzyme is secreted
Nakataea oryzae
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
Mn:Fe ratio is 1:0.05
Nakataea oryzae
Mn2+
manganese 9S-lipoxygenase, 9S-LOX contains catalytic manganese, Mn:protein ratio is about 0.2:1, while the Mn:Fe ratio is 1:0.05
Nakataea oryzae
additional information
the catalytic metal of the 9S-LOX is manganese and not iron
Nakataea oryzae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66000
-
x * 80000-90000, recombinant glycosylated enzyme, SDS-PAGE, x * 66000, recombinant deglycosylated enzyme, SDS-PAGE
Nakataea oryzae
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Pichia pastoris by hydrophobic interaction chromatography, dialfiltration, and gel filtration
Nakataea oryzae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Nakataea oryzae
-
Storage Stability (protein specific)
Storage Stability
Organism
purified recombinant 9S-MnLOX can be stored for several weeks at 4C with retained activity
Nakataea oryzae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(9E,11Z,15E)-octadeca-9,11,15-trienoic acid + O2
-
725658
Nakataea oryzae
?
-
-
-
?
(9E,11Z,15E)-octadeca-9,11,15-trienoic acid + O2
-
725658
Nakataea oryzae CBS 288.54
?
-
-
-
?
(9Z,12Z)-octadeca-9,12-dienoic acid + O2
alpha-linoleate is converted via two intermediates, (9Z,11S,12Z)-11-hydroperoxy-9,12-octadecenoate and (9Z,11E,13R)-13-hydroperoxy-9,12-octadecadienoate, which are isomerized to the end product, probably after oxidation to peroxyl radicals, beta-fragmentation, and oxygen insertion at C-9
725658
Nakataea oryzae
(9S,10E,12Z)-9-hydroperoxy-octadeca-10,12-dienoate
-
-
-
?
(9Z,12Z)-octadeca-9,12-dienoic acid + O2
alpha-linoleate is converted via two intermediates, (9Z,11S,12Z)-11-hydroperoxy-9,12-octadecenoate and (9Z,11E,13R)-13-hydroperoxy-9,12-octadecadienoate, which are isomerized to the end product, probably after oxidation to peroxyl radicals, beta-fragmentation, and oxygen insertion at C-9
725658
Nakataea oryzae CBS 288.54
(9S,10E,12Z)-9-hydroperoxy-octadeca-10,12-dienoate
-
-
-
?
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2
gamma-linoleate is oxidized at C-9, C-11, and C-13
725658
Nakataea oryzae
?
-
-
-
?
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2
gamma-linoleate is oxidized at C-9, C-11, and C-13
725658
Nakataea oryzae CBS 288.54
?
-
-
-
?
additional information
cf. EC 1.13.11.45, LC- and GC-MS analysis product analysis, overview. Authentic (11S)-hydroperoxyoctadecadienoate is rapidly transformed to 9-hydroperoxyoctadecadienoate by 9S-MnLOX, and only traces of 13-hydroperoxyoctadecadienoate. 9S-MnLOX also partly transforms authentic (13R)-hydroperoxyoctadecadienoate to (9S)-hydroperoxyoctadecadienoate
725658
Nakataea oryzae
?
-
-
-
-
additional information
cf. EC 1.13.11.45, LC- and GC-MS analysis product analysis, overview. Authentic (11S)-hydroperoxyoctadecadienoate is rapidly transformed to 9-hydroperoxyoctadecadienoate by 9S-MnLOX, and only traces of 13-hydroperoxyoctadecadienoate. 9S-MnLOX also partly transforms authentic (13R)-hydroperoxyoctadecadienoate to (9S)-hydroperoxyoctadecadienoate
725658
Nakataea oryzae CBS 288.54
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 80000-90000, recombinant glycosylated enzyme, SDS-PAGE, x * 66000, recombinant deglycosylated enzyme, SDS-PAGE
Nakataea oryzae
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at
Nakataea oryzae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.3
-
(9Z,12Z)-octadeca-9,12-dienoic acid
pH 9.0, 22C
Nakataea oryzae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Nakataea oryzae
General Information
General Information
Commentary
Organism
evolution
9S-LOX contains catalytic manganese, and its sequence can be aligned with 77% identity to 13R-LOX with catalytic manganese lipoxygenase of the Take-all fungus. Alterations in the Sloane determinant of 9S-LOX and 13R-MnLOX with larger and smaller hydrophobic residues interconvert the regiospecifi c oxidation of 18:2 n-6, presumably by altering the substrate position in relation to oxygen insertion
Nakataea oryzae
additional information
9S-MnLOX catalyzes hydrogen abstraction at C-11 and oxygenation at C-9 and C-11 in a suprafacial manner in analogy with 13R-MnLOX, EC 1.13.11.45
Nakataea oryzae
General Information (protein specific)
General Information
Commentary
Organism
evolution
9S-LOX contains catalytic manganese, and its sequence can be aligned with 77% identity to 13R-LOX with catalytic manganese lipoxygenase of the Take-all fungus. Alterations in the Sloane determinant of 9S-LOX and 13R-MnLOX with larger and smaller hydrophobic residues interconvert the regiospecifi c oxidation of 18:2 n-6, presumably by altering the substrate position in relation to oxygen insertion
Nakataea oryzae
additional information
9S-MnLOX catalyzes hydrogen abstraction at C-11 and oxygenation at C-9 and C-11 in a suprafacial manner in analogy with 13R-MnLOX, EC 1.13.11.45
Nakataea oryzae
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.45
-
(9Z,12Z)-octadeca-9,12-dienoic acid
pH 9.0, 22C
Nakataea oryzae
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.45
-
(9Z,12Z)-octadeca-9,12-dienoic acid
pH 9.0, 22C
Nakataea oryzae
Other publictions for EC 1.13.11.B6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742627
Schiller
A dual positional specific li ...
Malus domestica
Hortic. Res.
2
15003-15015
2015
-
-
1
-
7
-
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1
1
-
-
4
-
4
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1
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2
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6
1
2
2
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2
2
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2
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8
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2
2
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4
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2
-
4
-
-
6
2
2
2
-
-
2
2
-
-
-
4
7
-
-
-
725658
Wennman
Secretion of two novel enzymes ...
Nakataea oryzae, Nakataea oryzae CBS 288.54
J. Lipid Res.
54
762-775
2013
-
-
1
-
2
1
-
1
1
3
1
-
-
5
-
-
1
-
-
1
-
1
8
1
1
-
-
1
1
-
-
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-
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-
1
-
-
2
1
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1
1
3
1
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1
-
1
-
1
8
1
1
-
-
1
1
-
-
-
-
2
2
-
1
1
725856
Jerneren
Linolenate 9R-dioxygenase and ...
Lasiodiplodia theobromae, Lasiodiplodia theobromae 2334, Lasiodiplodia theobromae CBS 117454, Lasiodiplodia theobromae CBS 122127
Lipids
47
65-73
2012
-
-
-
-
-
-
-
-
1
-
-
13
-
6
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1
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17
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1
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1
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13
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1
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17
-
-
-
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1
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1
1
-
-
-
724736
Chechetkin
Oxidation of glycerolipids by ...
Zea mays
Chem. Phys. Lipids
164
216-220
2011
-
-
1
-
1
-
-
-
-
-
-
3
-
2
-
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12
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1
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1
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2
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3
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-
12
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
702431
Palmieri-Thiers
A lipoxygenase with dual posit ...
Olea europaea
Biochim. Biophys. Acta
1791
339-346
2009
-
-
1
-
-
-
2
2
1
-
2
-
-
1
-
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1
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1
1
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2
1
-
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2
1
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2
1
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1
-
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2
2
2
1
-
2
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1
-
1
1
-
2
1
-
-
-
2
1
-
-
1
-
1
1
-
2
2
706122
Hornung
Identification of an amino aci ...
Momordica charantia
Phytochemistry
69
2774-2780
2008
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-
1
-
2
-
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-
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1
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1
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1
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1
1
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1
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1
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2
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1
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1
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
706252
Wang
A novel lipoxygenase gene from ...
Oryza sativa
Plant Mol. Biol.
66
401-414
2008
-
-
1
-
-
-
-
-
-
-
1
1
-
5
-
-
-
-
-
3
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
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-
-
-
3
-
-
2
1
-
-
-
-
1
-
-
-
2
1
1
2
-
-
674388
Cho
Biochemical characterization o ...
Zea mays
J. Biochem. Mol. Biol.
40
100-106
2007
-
-
-
-
-
-
1
-
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1
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1
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706289
Veronico
A novel lipoxygenase in pea ro ...
Pisum sativum
Plant Physiol.
141
1045-1055
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
3
1
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1
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1
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-
-
-
-
-
1
1
-
3
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
703538
Santino
Biochemical and molecular char ...
Corylus avellana
Eur. J. Biochem.
270
4365-4375
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
1
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1
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1
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8
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706250
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A peanut seed lipoxygenase res ...
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702394
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Soybean lipoxygenase-1 enzymic ...
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