BRENDA - Enzyme Database show
show all sequences of 1.13.11.B6

A novel lipoxygenase in pea roots. Its function in wounding and biotic stress

Veronico, P.; Giannino, D.; Melillo, M.T.; Leone, A.; Reyes, A.; Kennedy, M.W.; Bleve-Zacheo, T.; Plant Physiol. 141, 1045-1055 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Pichia pastoris
Pisum sativum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
94000
-
x * 94000, SDS-PAGE
Pisum sativum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pisum sativum
Q14ST9
subsp. transcaucasicum Govorov
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
LOXN2 message is widespread in the cortex and endodermis of healthy roots, but specifically localized at high level in the cells bordering the nematode-induced syncytia of infected roots. In uninfected roots, the transcript is visualized in cortical and endodermal cells. In resistant infected roots, faint and infrequent signal spots are observed in the cortex, but LOXN2 message is abundant in the outer cells surrounding the vascular cylinder, in those cells injured by nematode penetration and those flanking the induced syncytia. In susceptible infected roots, the transcript is detected in cortical cells damaged by nematode invasion and undergoing necrosis, and in parenchymatous cells of the vascular cylinder strictly related to syncytia development
Pisum sativum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.35
-
activity with linoleate
Pisum sativum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arachidonate + O2
specific activity is 13% of the activity with linoleate
706289
Pisum sativum
?
products not determined
-
-
?
linoleate + O2
-
706289
Pisum sativum
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate
the ratio of 9-hydroperoxy-(10E,12Z)-octadecadienoate to 13-hydroperoxy-(10E,12Z)-octadecadienoate is 3:1
-
-
?
linolenate + O2
specific activity is 17% of the activity with linoleate. The R/S stereoconfiguration of the product is not determined
706289
Pisum sativum
?
products not determined
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 94000, SDS-PAGE
Pisum sativum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Pichia pastoris
Pisum sativum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
94000
-
x * 94000, SDS-PAGE
Pisum sativum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
LOXN2 message is widespread in the cortex and endodermis of healthy roots, but specifically localized at high level in the cells bordering the nematode-induced syncytia of infected roots. In uninfected roots, the transcript is visualized in cortical and endodermal cells. In resistant infected roots, faint and infrequent signal spots are observed in the cortex, but LOXN2 message is abundant in the outer cells surrounding the vascular cylinder, in those cells injured by nematode penetration and those flanking the induced syncytia. In susceptible infected roots, the transcript is detected in cortical cells damaged by nematode invasion and undergoing necrosis, and in parenchymatous cells of the vascular cylinder strictly related to syncytia development
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.35
-
activity with linoleate
Pisum sativum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
arachidonate + O2
specific activity is 13% of the activity with linoleate
706289
Pisum sativum
?
products not determined
-
-
?
linoleate + O2
-
706289
Pisum sativum
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate
the ratio of 9-hydroperoxy-(10E,12Z)-octadecadienoate to 13-hydroperoxy-(10E,12Z)-octadecadienoate is 3:1
-
-
?
linolenate + O2
specific activity is 17% of the activity with linoleate. The R/S stereoconfiguration of the product is not determined
706289
Pisum sativum
?
products not determined
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 94000, SDS-PAGE
Pisum sativum
Expression
Organism
Commentary
Expression
Pisum sativum
LOXN2 transcription is monitored in roots after mechanical injury and during nematode infection. The message peaks at 3 and 24 h after wounding in both genotypes and is more abundant in the resistant than in the susceptible pea. In nematode-infected roots, transcription of several LOX genes is triggered except LOXN2, which is repressed in both genotypes
down
General Information
General Information
Commentary
Organism
physiological function
LOXN2 may be involved in late mechanisms of host resistance
Pisum sativum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
LOXN2 may be involved in late mechanisms of host resistance
Pisum sativum
Expression (protein specific)
Organism
Commentary
Expression
Pisum sativum
LOXN2 transcription is monitored in roots after mechanical injury and during nematode infection. The message peaks at 3 and 24 h after wounding in both genotypes and is more abundant in the resistant than in the susceptible pea. In nematode-infected roots, transcription of several LOX genes is triggered except LOXN2, which is repressed in both genotypes
down
Other publictions for EC 1.13.11.B6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Schiller
A dual positional specific li ...
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2
15003-15015
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1
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7
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1
1
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4
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4
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1
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6
1
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2
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2
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4
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2
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4
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6
2
2
2
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2
2
-
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4
7
-
-
-
725658
Wennman
Secretion of two novel enzymes ...
Nakataea oryzae, Nakataea oryzae CBS 288.54
J. Lipid Res.
54
762-775
2013
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-
1
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2
1
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1
1
3
1
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5
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1
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8
1
1
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1
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1
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1
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1
8
1
1
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1
1
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2
2
-
1
1
725856
Jerneren
Linolenate 9R-dioxygenase and ...
Lasiodiplodia theobromae, Lasiodiplodia theobromae 2334, Lasiodiplodia theobromae CBS 117454, Lasiodiplodia theobromae CBS 122127
Lipids
47
65-73
2012
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1
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13
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6
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17
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13
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1
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17
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1
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1
1
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724736
Chechetkin
Oxidation of glycerolipids by ...
Zea mays
Chem. Phys. Lipids
164
216-220
2011
-
-
1
-
1
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3
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2
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12
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1
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1
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2
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3
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12
-
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1
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-
1
1
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-
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702431
Palmieri-Thiers
A lipoxygenase with dual posit ...
Olea europaea
Biochim. Biophys. Acta
1791
339-346
2009
-
-
1
-
-
-
2
2
1
-
2
-
-
1
-
-
1
-
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1
1
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2
1
-
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-
2
1
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2
1
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1
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2
2
2
1
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2
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1
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1
1
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2
1
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-
2
1
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-
1
-
1
1
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2
2
706122
Hornung
Identification of an amino aci ...
Momordica charantia
Phytochemistry
69
2774-2780
2008
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1
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2
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1
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1
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1
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1
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1
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1
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2
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1
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1
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1
1
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1
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706252
Wang
A novel lipoxygenase gene from ...
Oryza sativa
Plant Mol. Biol.
66
401-414
2008
-
-
1
-
-
-
-
-
-
-
1
1
-
5
-
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3
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2
1
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1
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1
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1
1
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3
-
-
2
1
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-
-
1
-
-
-
2
1
1
2
-
-
674388
Cho
Biochemical characterization o ...
Zea mays
J. Biochem. Mol. Biol.
40
100-106
2007
-
-
-
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1
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1
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1
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706289
Veronico
A novel lipoxygenase in pea ro ...
Pisum sativum
Plant Physiol.
141
1045-1055
2006
-
-
1
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1
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1
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1
1
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3
1
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1
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1
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1
1
-
3
1
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-
-
-
-
-
-
-
1
1
1
1
-
-
703538
Santino
Biochemical and molecular char ...
Corylus avellana
Eur. J. Biochem.
270
4365-4375
2003
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-
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1
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1
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1
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1
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706251
Kim
Dual positional specificity an ...
Zea mays
Plant Mol. Biol.
52
1203-1213
2003
-
-
1
-
-
-
-
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
1
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396190
Hughes
Probing a novel potato lipoxyg ...
Solanum tuberosum
Biochem. J.
353
345-355
2001
-
-
1
-
3
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1
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7
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1
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1
1
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2
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1
1
1
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1
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3
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1
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1
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1
1
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2
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1
1
1
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1
1
703535
Hughes
Mutagenesis and modelling of l ...
Pisum sativum
Eur. J. Biochem.
268
1030-1040
2001
-
-
1
-
8
-
-
1
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1
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4
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1
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1
1
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1
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1
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8
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1
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1
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1
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1
1
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1
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-
1
1
706250
Burow
A peanut seed lipoxygenase res ...
Arachis hypogaea
Plant Mol. Biol.
42
689-701
2000
-
-
1
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-
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-
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4
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4
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1
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1
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4
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1
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1
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1
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396184
Hughes
-
Characterization of authentic ...
Pisum sativum
Biochem. J.
333
33-43
1998
-
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1
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3
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4
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2
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1
2
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1
4
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2
1
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3
3
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4
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2
2
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3
3
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3
3
706645
Skrzypczak-Jankun
Structure of soybean lipoxygen ...
Glycine max
Proteins
29
15-31
1997
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1
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702394
Gardner
Soybean lipoxygenase-1 enzymic ...
Glycine max
Biochim. Biophys. Acta
1001
274-281
1989
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